The septohippocampal cholinergic system and spatial working memory in the Morris water maze
The septohippocampal cholinergic system and spatial working memory in the Morris water maze
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DOI：
10.1016/j.bbr.2005.10.008
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发表时间：
2006-03-15
影响因子：
2.7
通讯作者：
Pizzo, DP
中科院分区：
文献类型：
--
作者：
Frielingsdorf, H;Thal, LJ;Pizzo, DP
本研究的目的是确定是否系统优化的Morris水迷宫（mwm）测试参数可以揭示空间工作记忆中的隔海马胆碱能系统的重要作用。年轻的成年大鼠使用192 IgG-皂草素双侧注入内侧隔损伤。通过胆碱乙酰转移酶（ChAT）活性和免疫组织化学测定，病变接近完成。行为测试分三个阶段进行。首先，损伤和未损伤的大鼠在mwm中进行训练，重点是工作记忆，每天使用新的平台位置进行测试。在第二阶段，最佳位置重新测试，增加试验间隔（ITI）。在第三阶段，脑室内注射神经生长因子（NGF），以增强未受损大鼠的胆碱能活性，并可能进一步分离组的性能。无论是标准或增加ITI导致组间空间工作记忆的一致显着差异。此外，神经生长因子治疗也未能诱导行为表现的显着差异。总之，尽管海马ChAT损失超过90%，并使用最佳测试参数和NGF治疗，但无法显示由mwm评估的工作记忆损伤。(c)2005 Elsevier B. V.保留所有权利。
The objective of the present study was to determine whether a systematic optimization of Morris water maze (mwm) testing parameters could reveal a significant role of the septohippocampal cholinergic system in spatial working memory. Young adult rats were lesioned using 192 IgG-saporin infused bilaterally into the medial septum. Lesions were near complete as measured by choline acetyltransferase (ChAT) activity and immunohistochemistry. Behavioral testing was performed in three phases. In the first, lesioned and unlesioned rats were trained in the mwm focusing on working memory, which was tested using novel platform locations daily. In the second phase, the optimal locations were retested with increasing intertrial intervals (ITI). In the third phase, intracerebroventricular infusions of nerve growth factor (NGF) were employed to enhance cholinergic activity of the unlesioned rats and potentially further separate group performance. Neither the standard or increased ITI resulted in a consistent significant difference in spatial working memory between groups. In addition, NGF treatment also failed to induce a significant difference in behavioral performance. In conclusion, impairments in working memory as assessed by the mwm could not be revealed despite a greater than 90% loss of hippocampal ChAT and the use of optimal testing parameters and NGF treatment. (c) 2005 Elsevier B.V. All rights reserved.