Factors regulating the influence of melatonin on hippocampal evoked potentials: comparative studies on different strains of mice
Factors regulating the influence of melatonin on hippocampal evoked potentials: comparative studies on different strains of mice
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DOI：
10.1016/s0006-8993(02)02752-x
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发表时间：
2002-08
期刊：
影响因子：
2.9
通讯作者：
Y. El-Sherif;M. V. Hogan;Jene Tesoriero;A. Wieraszko
中科院分区：
文献类型：
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作者：
Y. El-Sherif;M. V. Hogan;Jene Tesoriero;A. Wieraszko
在小鼠海马脑片中，评价了调节褪黑素对海马谷氨酸能系统影响的因素。海马锥体神经元对褪黑素（Sigma）的敏感性在切片制备后2h最高，然后随时间下降。这种对褪黑激素的敏感性模式与褪黑激素与其受体的结合减少密切相关。从老年动物身上获得的切片在整个潜伏期内对褪黑激素保持敏感。大多数评价褪黑激素对海马诱发电位影响的实验在切片制备后2小时内进行。褪黑激素的效果是双相的：一个初始的抑郁症的潜力，其次是恢复/放大阶段。恢复期不是褪黑激素分解的结果。褪黑激素的作用在三种不同的小鼠品系中是相似的：CD-1、C57 J/B6和Swiss韦伯斯特。虽然来自另一家供应商（Regis）的褪黑激素给出了类似的结果，但它在低得多的浓度下有效。在从CD-1光剥夺小鼠获得的切片中，对褪黑激素的敏感性显着降低。因此，看来褪黑激素可以控制海马谷氨酸能系统以复杂的方式，这可能是由昼夜节律调节。这可能会影响海马体中的记忆形成。
Factors regulating the influence of melatonin on the hippocampal glutamergic system in mouse hippocampal slices were evaluated. The sensitivity of hippocampal pyramidal neurons to melatonin (Sigma) was highest at 2 h following slice preparation and then declined with time. This pattern of sensitivity to melatonin correlated well with a reduced binding of melatonin to its receptors. The slices obtained from older animals remained sensitive to melatonin through the entire incubation period. Most of the experiments evaluating the influence of melatonin on hippocampal evoked potentials were performed within 2 h following slice preparation. The effect of melatonin was biphasic: an initial depression of the potential was followed by a recovery/amplification phase. The recovery phase was not a result of melatonin decomposition. The effect of melatonin was similar in three different strains of mice tested: CD-1, C57J/B6, and Swiss Webster. While the melatonin from another vendor (Regis) gave similar results, it was effective at much lower concentrations. In slices obtained from CD-1 light-deprived mice, the sensitivity to melatonin was significantly reduced. Thus, it appears that melatonin may control the hippocampal glutamergic system in a complex manner, which may be regulated by the circadian rhythm. This may influence memory formation in the hippocampus.