Antimicrobial Resistance Patterns and Integron Carriage of Escherichia coli Isolates Causing Community-Acquired Infections in Turkey
Antimicrobial Resistance Patterns and Integron Carriage of Escherichia coli Isolates Causing Community-Acquired Infections in Turkey
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DOI：
10.3343/alm.2014.34.2.139
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发表时间：
2014-03-01
影响因子：
4.9
通讯作者：
Sandalli, Cemal
中科院分区：
文献类型：
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作者：
Copur-Cicek, Aysegul;Ozgumus, Osman Birol;Sandalli, Cemal
我们的目的是观察耐药模式和整合子携带的大肠埃希菌分离株引起社区获得性感染。268个E。大肠杆菌菌株从土耳其里泽第82届国立医院不同综合诊所的各种感染的门诊患者中获得。使用纸片扩散法测试对抗菌剂的敏感性。使用特异性引物的PCR检查整合子的存在。通过测序确认阳性PCR结果。使用肉汤交配法进行接合试验。使用寡核苷酸引物BOXA 1 R进行基因外回文-FOR。对氨苄西林、甲氧苄啶/磺胺甲恶唑、四环素的耐药率分别为50.6%、33.5%、36.8%。无菌株对阿米卡星耐药。70个分离株为intll基因阳性，其中49个携带基因盒。11个分离株为int 12基因阳性，其中8个携带基因盒。7个基因盒（dfrA 1、dfrA 5、dfrA 7、dfrA 17、aadA 1、aadA 5和sat 2）主要存在于整合子中。我们在两株大肠杆菌中检测到含有整合子的接合质粒。大肠杆菌菌株。BOX-FOR指纹图谱显示4个菌株聚类，表明它们具有克隆相关性。携带1类和2类整合子的菌株之间没有明显的关系。我们的结论是整合子广泛存在于遗传变异的大肠杆菌中。本研究旨在观察引起社区获得性感染的大肠埃希菌分离株的耐药模式和整合子携带情况。268个E。大肠杆菌菌株从土耳其里泽第82届国立医院不同综合诊所的各种感染的门诊患者中获得。使用纸片扩散法测试了对抗菌药物的敏感性。使用特异性引物的PCR检查整合子的存在。通过测序确认阳性PCR结果。使用肉汤交配法进行接合试验。使用寡核苷酸引物BOXA 1 R进行基因外回文-FOR。对氨苄西林、甲氧苄啶/磺胺甲恶唑、四环素的耐药率分别为50.6%、33.5%、36.8%。无菌株对阿米卡星耐药。70个分离株为intll基因阳性，其中49个携带基因盒。11个分离株为int 12基因阳性，其中8个携带基因盒。7个基因盒（dfrA 1、dfrA 5、dfrA 7、dfrA 17、aadA 1、aadA 5和sat 2）主要存在于整合子中。我们在两株大肠杆菌中检测到含有整合子的接合质粒。大肠杆菌菌株。BOX-FOR指纹图谱显示4个菌株聚类，表明它们具有克隆相关性。携带1类和2类整合子的菌株之间没有明显的关系。我们的结论是整合子广泛存在于遗传变异的大肠杆菌中。大肠杆菌菌株，并将继续介导耐药基因在社区中的传播。
We aimed to observe antimiCrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections. Two hundred sixty-eight E. coli strains were obtained from outpatients with various infections at different polyclinics at the 82nd Year of State Hospital in Rize, Turkey. Susceptibility to antimicrobials was tested using a disk diffusion method. The presence of integrons was examined using PCR with specific primers. Positive PCR results were confirmed by sequencing. A broth mating method was used for conjugation assays. Extragenic palindromic-FOR was performed using the oligonucleotide primer BOXA1R. Resistance frequency for ampicillin,trimethoprim/sulfamethoxazole, and tetracycline was determined as 50.6%, 33.5%, and 36.8% respectively. No strains were resistant to amikacin. Seventy isolates were positive for the intll gene, of which 49 carried gene cassettes. Eleven isolates were positive for the int12 gene, eight of swhich carried gene cassettes. Seven gene cassettes (dfrAl, dfrA5, dfrA7, dfrA17, aadAl, aadA5, and sat2) were predominantly harbored in integrons. We detected conjugative plasmids harboring integrons in two E. coli strains. Four strain clusters were yielded by BOX-FOR fingerprints showing that they were clonally related. No apparent relationship occurred among class 1 and 2 integron-carrying strains. We conclude that integrons are widespread in genetically variable E. coli strains and will continue to mediate dissemination of resistance genes in the community.We aimed to observe antimiCrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections. Two hundred sixty-eight E. coli strains were obtained from outpatients with various infections at different polyclinics at the 82nd Year of State Hospital in Rize, Turkey. Susceptibility to antimicrobials was tested using a disk diffusion method. The presence of integrons was examined using PCR with specific primers. Positive PCR results were confirmed by sequencing. A broth mating method was used for conjugation assays. Extragenic palindromic-FOR was performed using the oligonucleotide primer BOXA1R. Resistance frequency for ampicillin, trimethoprim/sulfamethoxazole, and tetracycline was determined as 50.6%, 33.5%, and 36.8% respectively. No strains were resistant to amikacin. Seventy isolates were positive for the intll gene, of which 49 carried gene cassettes. Eleven isolates were positive for the int12 gene, eight of swhich carried gene cassettes. Seven gene cassettes (dfrAl, dfrA5, dfrA7, dfrA17, aadAl, aadA5, and sat2) were predominantly harbored in integrons. We detected conjugative plasmids harboring integrons in two E. coli strains. Four strain clusters were yielded by BOX-FOR fingerprints showing that they were clonally related. No apparent relationship occurred among class 1 and 2 integron-carrying strains. We conclude that integrons are widespread in genetically variable E. coli strains and will continue to mediate dissemination of resistance genes in the community.