THYROID HORMONE-DEPENDENT TRANSCRIPTIONAL REGULATION OF EXOGENOUS GENES TRANSFERRED INTO XENOPUS TADPOLE MUSCLE IN-VIVO
THYROID HORMONE-DEPENDENT TRANSCRIPTIONAL REGULATION OF EXOGENOUS GENES TRANSFERRED INTO XENOPUS TADPOLE MUSCLE IN-VIVO
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DOI：
10.1073/pnas.90.15.7322
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发表时间：
1993-08-01
影响因子：
11.1
通讯作者：
DEMENEIX, B
中科院分区：
文献类型：
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作者：
DELUZE, A;SACHS, L;DEMENEIX, B
两栖动物的变态特征是甲状腺激素引起的剧烈变化，包括尾部退化。为了研究甲状腺激素对基因转录的影响，在早期阶段的尾巴吸收，我们将外源基因直接注入到非洲爪蟾蝌蚪的尾部骨骼肌，并遵循其在体内的表达。基因表达既强又可重复，并且与注射的DNA量相关。此外，表达持续，只要动物被阻断在变态前期的抗甲状腺药物（长达4个月）。甲状腺激素依赖性转录的影响进行了研究，通过使用回文甲状腺激素反应元件连接到氯霉素乙酰转移酶报告基因。通过使用组成型表达的荧光素酶构建体，将报告基因表达针对转染效率进行标准化。生理浓度的3，5，3 '-三碘-L-甲状腺原氨酸（1 nM）作用120小时，使转录增加5倍（P
Metamorphosis in amphibians is marked by dramatic thyroid hormone-induced changes that include tail regression. To examine thyroid hormone effects on gene transcription during the early stages of tail resorption, we injected exogenous genes directly into the caudal skeletal muscle of Xenopus tadpoles and followed their expression in vivo. Gene expression was both strong and reproducible, and it correlated with the amount of DNA injected. Moreover, expression continued as long as the animals were blocked in prometamorphosis by antithyroid drugs (for up to 4 months). Thyroid hormone-dependent effects on transcription were examined by using a palindromic thyroid hormone response element linked to a chloramphenicol acetyltransferase reporter gene. Reporter gene expressions were normalized for transfection efficiency by using a constitutively expressed luciferase construct. Physiological concentrations of 3,5,3' triiodo-L-thyronine (1 nM), applied for 120 hr, produced a 5-fold increase in transcription (P