Liquid chromatography with atmospheric pressure chemical ionization and electrospray ionization mass spectrometry of flavonoids with triple-quadrupole and ion-trap instruments
Liquid chromatography with atmospheric pressure chemical ionization and electrospray ionization mass spectrometry of flavonoids with triple-quadrupole and ion-trap instruments
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DOI：
10.1016/s0021-9673(02)01868-x
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发表时间：
2003-01-10
影响因子：
4.1
通讯作者：
Brinkman, UAT
中科院分区：
文献类型：
--
作者：
de Rijke, E;Zappey, H;Brinkman, UAT
以15种黄酮类化合物为被试化合物，比较了LC-MS、多MS(MS“)和串联操作(常压化学电离、电喷雾电离、正负电离)四种模式在三重四极杆和离子捕捉仪上的分析性能。使用了两种有机改性剂，甲醇和乙腈，以及两种缓冲液，乙酸铵和甲酸铵。总体而言，在负离子模式下使用APCI获得了最好的响应，两台仪器的信号强度和质谱学特征没有显著差异。以甲醇-甲酸铵(pH 4.0)为洗脱液效果最好。在最佳条件下，在负向进样模式下，可获得0.10-30 mg/L的全扫描检测限。这里需要强调的是，在分析物之间和电离模式之间都发现了高达2阶的响应差异。这意味着，在解释有关筛选真实样本的数据时，应该非常谨慎。在离子陷阱的“MS”谱或三四极杆的串联MS谱中观察到的主要碎裂大致相同。前一种方法的优点是增加了确定前体-->；产品离子关系的可能性。(C)2002 Elsevier Science B.V.保留所有权利。
With 15 flavonoids as test compounds, the analytical performance of four modes of LC-MS, multiple MS (MS") and tandem MS operation (atmospheric pressure chemical ionization (APCI), electrospray ionization, positive and negative ionization) was compared for two mass spectrometers, a triple-quadrupole and an ion-trap instrument. Two organic modifiers, methanol and acetonitrile, and two buffers, ammonium acetate and ammonium formate, were used. In general, the use of APCI in the negative ion mode gave the best response, with the signal intensities and the mass-spectral characteristics not differing significantly between the two instruments. The best results were obtained when methanol-ammonium formate (pH 4.0) was used as LC eluent. Under optimum conditions full-scan limits of detection of 0.1-30 mg/l were achieved in the negative APCI mode. Here it needs to be emphasized that up to 2-order response differences were found both between analytes and between modes of ionization. This implies that one should be very cautious when interpreting data on the screening of real-life samples. The main fragmentations observed in the MS" spectra on the ion-trap, or the tandem MS spectra on the triple-quadrupole were generally the same. The advantage of the former approach is the added possibility to ascertain precursor-->product ion relationships. (C) 2002 Elsevier Science B.V. All rights reserved.