Production of arrays of chemically distinct nanolitre plugs via repeated splitting in microfluidic devices
Production of arrays of chemically distinct nanolitre plugs via repeated splitting in microfluidic devices
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DOI：
10.1039/b604993a
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发表时间：
2006-01-01
期刊：
影响因子：
6.1
通讯作者：
Ismagilov, Rustem F.
中科院分区：
文献类型：
--
作者：
Adamson, David N.;Mustafi, Debarshi;Ismagilov, Rustem F.
本文报道了一种制备具有不同化学成分的纳米管塞阵列的方法。在大规模生物筛选中使用基于插头的微流体的主要限制之一是在纳米级上制造化学上不同的插头阵列的困难。这里，使用具有多个串联的T型结的微流控器件，将大(类似320 NL)插头的单个输入阵列拆分，以产生16个较小(类似于20 NL)插头的输出阵列；这些阵列的组成和配置与输入的相同。本文展示了如何利用T结微通道几何结构中的插头的被动分解来从单个大体积阵列中产生一组用于化学筛选的较小体积的输出阵列。给出了一个简单的理论描述，描述了裂解作为毛细管数、毛细压力、流道总压差和几何流体阻力的函数。通过考虑这些因素，可以消除裂解过程中的堵塞合并和堵塞污染，并可以保持裂解的对称性。此外，还利用基于阀门和基于体积的方法实现了单出口分流装置，以协调输出阵列的释放。从所提出的分裂方法中获得了包含商业稀疏矩阵屏幕的塞子阵列，并将这些阵列用于蛋白质结晶实验。本文所介绍的技术有助于实现高通量的化学和生物筛选。
This paper reports a method for the production of arrays of nanolitre plugs with distinct chemical compositions. One of the primary constraints on the use of plug-based microfluidics for large scale biological screening is the difficulty of fabricating arrays of chemically distinct plugs on the nanolitre scale. Here, using microfluidic devices with several T-junctions linked in series, a single input array of large (similar to 320 nL) plugs was split to produce 16 output arrays of smaller (similar to 20 nL) plugs; the composition and configuration of these arrays were identical to that of the input. This paper shows how the passive break-up of plugs in T-junction microchannel geometries can be used to produce a set of smaller-volume output arrays useful for chemical screening from a single large-volume array. A simple theoretical description is presented to describe splitting as a function of the Capillary number, the capillary pressure, the total pressure difference across the channel, and the geometric fluidic resistance. By accounting for these considerations, plug coalescence and plug -plug contamination can be eliminated from the splitting process and the symmetry of splitting can be preserved. Furthermore, single-outlet splitting devices were implemented with both valve-and volume-based methods for coordinating the release of output arrays. Arrays of plugs containing commercial sparse matrix screens were obtained from the presented splitting method and these arrays were used in protein crystallization trials. The techniques presented in this paper may facilitate the implementation of high-throughput chemical and biological screening.