Hemokinin-1 has Substance P-like function in U-251 MG astrocytoma cells: A pharmacological and functional study
Hemokinin-1 has Substance P-like function in U-251 MG astrocytoma cells: A pharmacological and functional study
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DOI：
10.1016/j.jneuroim.2005.03.016
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发表时间：
2005-07-01
影响因子：
3.3
通讯作者：
Paige, CJ
中科院分区：
文献类型：
--
作者：
Berger, A;Paige, CJ
P物质（SP）触发星形细胞瘤细胞的反应，这被认为对增殖和神经调节活性很重要。在本研究中，我们比较了SP与新型快速激肽hemkinin -1 （HK-1）在人星形细胞瘤细胞系U-251 MG中的作用。我们发现U-251 MG细胞表达高水平的神经激肽-1 （NK-1）受体。I-125-SP和I-125-mHK-1对这些受体的结合亲和力在相似的亚纳摩尔范围内。HK-1和SP刺激Ca2+动员，诱导细胞因子mRNA表达增加。一种特异性NK-1受体拮抗剂阻断了观察到的作用。我们得出结论，SP和hk -1诱导的反应没有质的差异，这表明这两种肽都通过NK-1受体在U-251 MG细胞中起作用。此外，我们在胶质瘤中发现了tac4mrna的表达，表明HK-1可能参与胶质瘤生物学。(C) 2005 Elsevier B.V.版权所有
Substance P (SP) triggers responses in astrocytoma cells, which are considered important for proliferation and neuroinununomodulatory activity. In this study, we compared the effects of SP with those of the novel tachykinin Hemokinin-1 (HK-1) in the human astrocytoma cell line U-251 MG. We show that U-251 MG cells express high levels of Neurokinin-1 (NK-1) receptors. The binding affinities of I-125-SP and I-125-mHK-1 to these receptors were in a similar, subnanomolar range. HK-1 and SP stimulated Ca2+ mobilization and induced increased cytokine mRNA expression. A specific NK-1 receptor antagonist blocked the observed effects. We conclude that there are no qualitative differences in SP and HK-1-evoked responses, suggesting that both peptides act through NK-1 receptors in U-251 MG cells. Moreover, we show TAC4 mRNA expression in gliomas, indicating a possible involvement of HK-1 in glioma biology. (C) 2005 Elsevier B.V. All rights reserved.