Transient over-expression of NGFI-A gene suppresses NGF-induced neurite outgrowth in PC12 cells
Transient over-expression of NGFI-A gene suppresses NGF-induced neurite outgrowth in PC12 cells
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DOI：
10.1097/00001756-200004070-00021
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发表时间：
2000-04-07
期刊：
影响因子：
1.7
通讯作者：
Noda, M
中科院分区：
文献类型：
--
作者：
Matsumoto, T;Akiyama, N;Noda, M
使用诱导型基因表达系统（Tet-ON 系统），检查了 NGFI-A 基因在 PC12 细胞神经元分化过程中的作用。当 NGFI-A 短暂过表达时，没有观察到对细胞增殖或神经突生长的明显影响。然而有趣的是，NGFI-A 过度表达导致 NGF 诱导的神经突生长显着延迟。对 v-K-ras 诱导的神经突生长也观察到类似的抑制作用。这些结果提出了 NGFI-A 蛋白可能在 NGF 信号传导中发挥某些负面作用的可能性。 NeuroReport 11：1001-1005 (C) 2000 Lippincott Williams & Wilkins。
Using an inducible gene expression system (Tet-ON system), the role of NGFI-A gene during the neuronal differentiation of PC12 cells was examined. When NGFI-A was transiently overexpressed, no obvious effects on cell proliferation or neurite outgrowth were observed. Interestingly, however, NGFI-A over-expression resulted in significant retardation in NGF-induced neurite outgrowth. Similar suppressive effects were observed also on the v-K-ras-induced neurite outgrowth. These results raise the possibility that NGFI-A protein may play some negative role in NGF signaling. NeuroReport 11:1001-1005 (C) 2000 Lippincott Williams & Wilkins.