Two dimensional electrophoresis of the exo-proteome produced from community acquired methicillin resistant Staphylococcus aureus belonging to clonal complex 80
Two dimensional electrophoresis of the exo-proteome produced from community acquired methicillin resistant Staphylococcus aureus belonging to clonal complex 80
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DOI：
10.1016/j.micres.2013.03.004
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发表时间：
2013-01-01
影响因子：
6.7
通讯作者：
Yamamoto, Tadashi
中科院分区：
文献类型：
--
作者：
Enany, Shymaa;Yoshida, Yutaka;Yamamoto, Tadashi
采用双向电泳（2DE）结合质谱技术对两株社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）CC 80菌株（ER 13和ER 21）分泌的胞外蛋白质组进行了分析。使用Progenesis SameSpots软件检测2种凝胶之间的共同点。从ER 13和ER 21的外切蛋白质组中分别解析了251和312个点。2DE重叠比较显示，共有59个位点。LC-MS/MS分析从这些点鉴定了57种蛋白质，包括约21%的细胞外蛋白质、48%的细胞质蛋白质、2%的细胞质膜蛋白质、2%的细胞壁蛋白质和26%的未知定位蛋白质。鉴定的蛋白质根据其基因本体（GO）注释分类为与24%毒力决定簇和毒素相似，与17%参与碳水化合物代谢相似，与14%参与环境应激相似，与12%与细胞分裂相关相似。从我们研究中使用的两种菌株的外产物中鉴定肠毒素B属于CC 80是有趣的。(C)2013年爱思唯尔有限公司。All rights reserved.
Two-dimensional electrophoresis (2DE) combined with mass spectrometry was used to characterize the exo-proteome secreted by two strains (ER13 and ER21) representing community acquired methicillin resistant Staphylococcus aureus (CA-MRSA) belonging to clonal complex 80 (CC80). Common spots were detected between the 2 gels using the Progenesis SameSpots software. Two hundred and fifty-one and 312 spots from the exo-proteome of ER13 and ER21 were resolved, respectively. 2DE overlap comparison showed that 59 spots were shared. LC-MS/MS analysis identified 57 proteins from these spots comprising about 21% extracellular, 48% cytoplasmic, 2% cytoplasmic membrane, 2% cell wall, and 26% with unknown localization. The identified proteins were classified with respect to their Gene Ontology (GO) annotation as similar to 24% virulence determinants and toxins, similar to 17% involved in carbohydrate metabolism, similar to 14% involved in environmental stress, and similar to 12% associated with cell division. The identification of the enterotoxin B from the exo-products of both strains used in our study, as belonging to CC80 was interesting. (C) 2013 Elsevier GmbH. All rights reserved.