Assessing the use of Quantitative Light-induced Fluorescence-Digital as a clinical plaque assessment
Assessing the use of Quantitative Light-induced Fluorescence-Digital as a clinical plaque assessment
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DOI：
10.1016/j.pdpdt.2015.12.002
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发表时间：
2016-03-01
影响因子：
3.3
通讯作者：
Kim, Baek-Il
中科院分区：
文献类型：
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作者：
Han, Sun-Young;Kim, Bo-Ra;Kim, Baek-Il
背景：本研究的目的是比较定量光诱导荧光数字（QLF-D）法检测的红色荧光斑块（RF斑块）面积与双色显示法检测的显示斑块面积之间的关系，并利用实时荧光定量pcr技术评估RF斑块的细菌组成。方法：选取50名健康受试者，对其前牙600个面面进行检查。在披露前后两次分别取QLF-D，并根据斑块百分比指数（PPI）计算RF斑块面积。揭露后，分析染色斑块面积，探讨与RF斑块面积的关系。采用Pearson相关和配对t检验对相关性进行评价。然后，从同一受试者身上获得RF和非RF斑块样本进行实时PCR检测。采用Wilcoxon sign rank检验比较10个菌斑样本中6个细菌的比例。结果：配对t检验显示，Delta R20时，蓝色染色斑块面积（9.3 +/- 9.2）与RF斑块面积（9.1 +/- 14.9,p = 0.80）具有显著的相似性，而红色染色斑块面积（31.6 +/- 20.9）与RF斑块面积存在差异（p < 0.0001）。此外，与非RF斑块相比，中间普雷沃氏菌和血管链球菌的细菌组成与RF斑块的相关性更大（p < 0.05）。结论：使用QLF-D的斑块评估方法具有检测成熟斑块的潜力，并且斑块面积与使用双色显示的蓝色染色区域相关。(C) 2015 Elsevier B.V.版权所有
Background: The aims of this study were to compare the relationship between red fluorescent plaque (RF plaque) area by Quantitative Light -induced Fluorescence-Digital (QLF-D) and disclosed plaque area by two-tone disclosure, and to assess the bacterial composition of the RF plaque by real time-PCR.Methods: Fifty healthy subjects were included and 600 facial surfaces of their anterior teeth were examined. QLF-D was taken on two separate occasions (before and after disclosing), and the RF plaque area was calculated based on Plaque Percent Index (PPI). After disclosing, the stained plaque area was analyzed to investigate the relationship with the RF plaque area. The relationship was evaluated using Pearson correlation and paired t-test. Then, the RF and non-red fluorescent (non-RF) plaque samples were obtained from the same subject for real-time PCR test. Total 10 plaque samples were compared the ratio of the 6 of bacteria using Wilcoxon signed rank test.Results: Regarding the paired t-test, the blue-staining plaque area (9.3 +/- 9.2) showed significantly similarity with the RF plaque area (9.1 +/- 14.9, p = 0.80) at Delta R20, however, the red-staining plaque area (31.6 +/- 20.9) presented difference from the RF plaque area (p < 0.0001). In addition, bacterial composition of Prevotella intermedia and Streptococcus anginosus was associated with substantially more the RF plaque than the non-RF plaque (p < 0.05).Conclusions: The plaque assessment method using QLF-D has potential to detect mature plaque, and the plaque area was associated with the blue-staining area using two-tone disclosure. (C) 2015 Elsevier B.V. All rights reserved.