Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA
Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA
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DOI：
10.1126/science.277.5324.383
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发表时间：
1997-07-18
期刊：
影响因子：
56.9
通讯作者：
Jansen, RP
中科院分区：
文献类型：
--
作者：
Long, RM;Singer, RH;Jansen, RP
产生不同基因表达模式的后代的细胞分裂是多细胞生物体发展的关键。在萌芽酵母中，母细胞而不是子细胞可以转换交配类型，因为它们选择性地表达HO内切酶基因。这种不对称是由于不稳定的转录抑制蛋白Ash1p在子细胞核中优先积累。这表明ASH1信使RNA(MRNA)通过依赖于肌动蛋白和肌球蛋白的过程优先积聚在子代细胞中。ASH1mRNA 3‘-非翻译区的顺式作用元件足以将嵌合RNA定位于子细胞。这些结果表明，mRNA的定位可能是真核生物谱系的早期特性。
Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells but not daughter cells can switch mating type because they selectively express the HO endonuclease gene. This asymmetry is due to the preferential accumulation of an unstable transcriptional repressor protein, Ash1p, in daughter cell nuclei. Here it is shown that ASH1 messenger RNA (mRNA) preferentially accumulates in daughter cells by a process that is dependent on actin and myosin. A cis-acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage.