Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
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DOI：
10.3109/17453674.2013.858289
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发表时间：
2013-12-01
影响因子：
3.7
通讯作者：
Scott, Alex
中科院分区：
文献类型：
--
作者：
Lundgreen, Kirsten;Lian, Oystein Bjerkestrand;Scott, Alex
背景和目的 肩袖撕裂与继发性肩袖肌肉病理有关，这对于肩袖修复的预后具有决定性作用。关于人类这些肌肉变化的早期组织学和免疫组织化学性质的信息很少。我们分析了冈上肌腱撕裂患者的肌肉活检。方法从 24 名接受关节镜下部分或全层冈上肌腱撕裂修复的患者中获取冈上肌活检。组织经过福尔马林固定和处理，用于组织学（用于评估脂肪浸润和其他退行性变化）或免疫组织化学（用于识别卫星细胞 (CD56+)、增殖细胞 (Ki67+) 和主要含有 1 型或 2 型肌球蛋白重链 (MHC) 的肌纤维）。通过形态计量学测定肌纤维直径以及 MHC1 和 MHC2 的相对含量。结果 两组患者（部分和全层撕裂）均出现退行性改变。全层撕裂患者卫星细胞密度降低，增殖细胞减少，MHC1+和MHC2+肌纤维萎缩，MHC1含量降低。解读与部分冈上肌腱撕裂相比，全层撕裂显示肌肉增殖能力显着降低，肌纤维萎缩，MHC1含量减少。
Background and purpose Rotator cuff tears are associated with secondary rotator cuff muscle pathology, which is definitive for the prognosis of rotator cuff repair. There is little information regarding the early histological and immunohistochemical nature of these muscle changes in humans. We analyzed muscle biopsies from patients with supraspinatus tendon tears.Methods Supraspinatus muscle biopsies were obtained from 24 patients undergoing arthroscopic repair of partial-or full-thickness supraspinatus tendon tears. Tissue was formalin-fixed and processed for histology (for assessment of fatty infiltration and other degenerative changes) or immunohistochemistry (to identify satellite cells (CD56+), proliferating cells (Ki67+), and myofibers containing predominantly type 1 or 2 myosin heavy chain (MHC)). Myofiber diameters and the relative content of MHC1 and MHC2 were determined morphometrically.Results Degenerative changes were present in both patient groups (partial and full-thickness tears). Patients with full-thickness tears had a reduced density of satellite cells, fewer proliferating cells, atrophy of MHC1+ and MHC2+ myofibers, and reduced MHC1 content.Interpretation Full-thickness tears show significantly reduced muscle proliferative capacity, myofiber atrophy, and loss of MHC1 content compared to partial-thickness supraspinatus tendon tears.