Experimental host range and histopathology of white spot syndrome virus (WSSV) infection in shrimp, prawns, crabs and lobsters from India
Experimental host range and histopathology of white spot syndrome virus (WSSV) infection in shrimp, prawns, crabs and lobsters from India
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DOI：
10.1046/j.1365-2761.1999.00162.x
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发表时间：
1999-05-01
影响因子：
2.5
通讯作者：
Krol, RM
中科院分区：
文献类型：
--
作者：
Rajendran, KV;Vijayan, KK;Krol, RM
通过将从印度东南海岸采集的受感染的斑节对虾（Penaeus monodon（Fabricius））中提取的白色斑点综合征病毒（WSSV）注射或饲喂5种虾、2种淡水对虾、4种螃蟹和3种龙虾进行了实验研究。所有受检物种均对该病毒易感。实验感染斑节对虾的临床症状和组织病理学特征与自然感染的斑节对虾相同。在注射WSSV的对虾中，在5-7天内观察到100%的累积死亡率，而在用感染组织喂养的对虾中，在7-9天内观察到100%的累积死亡率。两种泥蟹，Scylla sp.，感染后存活30天，无任何临床症状。这三种龙虾，龙虾属，罗氏沼虾（Macrobrachium rosenbergium（De Man））在感染后存活70天，无临床症状。然而，使用健康斑节对虾的生物测定和组织学显示，螃蟹、对虾和龙虾可能是WSSV的无症状携带者/储存宿主。这是第一份报告，建议通过组织学和生物测定证据的载体/水库这些主机的能力。本文还对实验感染的凡纳滨对虾的病毒的超微结构进行了研究。
Experimental studies were conducted by injecting or feeding white spot syndrome virus (WSSV) derived from infected shrimp, Penaeus monodon (Fabricius), collected from the south-east coast of India, to five species of shrimp, two species of freshwater prawns, four species of crabs and three species of lobsters. All species examined were susceptible to the virus. Experimental infections in the shrimp had the same clinical symptoms and histopathological characteristics as in naturally infected P. monodon. A cumulative mortality of 100% was observed within 5-7 days in shrimp injected with WSSV and 7-9 days in shrimp fed with infected tissue. Two species of mud crab, Scylla sp., survived the infection for 30 days without any clinical symptoms. All three species of lobsters, Panulirus sp., and the freshwater prawn, Macrobrachium rosenbergii (De Man), survived the infection for 70 days without clinical symptoms. However, bioassay and histology using healthy P. monodon revealed thar crabs, prawns and lobsters may act as asymptomatic carriers/reservoir hosts of WSSV. This is the first report to suggest the carrier/reservoir capacity of these hosts through histological and bioassay evidences. Ultrastructural derails of the virus in experimentally infected shrimp, P. vannamei, (Boone), were also studied.