Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways.
Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways.
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DOI：
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发表时间：
2012-09
影响因子：
2
通讯作者：
S. Miwa;N. Sugimoto;N. Yamamoto;T. Shirai;H. Nishida;K. Hayashi;H. Kimura;A. Takeuchi;K. Igarashi
中科院分区：
文献类型：
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作者：
S. Miwa;N. Sugimoto;N. Yamamoto;T. Shirai;H. Nishida;K. Hayashi;H. Kimura;A. Takeuchi;K. Igarashi
我们以前报道过咖啡因辅助化疗改善了恶性骨和软组织肿瘤如骨肉瘤的治疗。咖啡因通过各种途径影响肿瘤细胞，包括10号染色体上缺失的磷酸酶和张力蛋白同源物（PTEN）、AKT、Bcl-2相关X蛋白（BAX）、半胱天冬酶-3和p53，因此已被指示可用于治疗恶性肿瘤。采用WST-8法检测咖啡因对HOS骨肉瘤细胞增殖的影响，Western blot法检测咖啡因对活化B细胞核因子κ轻链增强子（NF-κB）、哺乳动物雷帕霉素靶蛋白（mTOR）和丝裂原活化蛋白激酶（MAPK）通路的影响。咖啡因抑制HOS细胞增殖，抑制NF-κB、AKT、mTOR/S6 K和ERK活性。我们的研究结果支持那些从以前的研究有关使用咖啡因治疗骨肉瘤。
We previously reported that caffeine-assisted chemotherapy improved the treatment of malignant bone and soft tissue tumours such as osteosarcoma. Caffeine affects tumour cells through various pathways, including phosphatase and tensin homolog deleted on chromosome 10 (PTEN), AKT, Bcl-2-associated X protein (BAX), caspase-3 and p53, and has therefore been indicated as being useful for the treatment of malignant tumours. Here, the effects of caffeine on the proliferation of HOS osteosarcoma cells were assessed by WST-8 assay, and the effects on the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), mammalian target of rapamycin (mTOR) and mitogen-activated protein kinase (MAPK) pathways were assessed by western blot analyses. Caffeine inhibited proliferation of HOS cells and suppressed NF-κB, AKT, mTOR/S6K and ERK activities. Our results support those from previous studies relating to the use of caffeine in the treatment of osteosarcoma.