Ion Release, Microstructural, and Biological Properties of iRoot BP Plus and ProRoot MTA Exposed to an Acidic Environment
Ion Release, Microstructural, and Biological Properties of iRoot BP Plus and ProRoot MTA Exposed to an Acidic Environment
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暴露于酸性环境中的 iRoot BP Plus 和 ProRoot MTA 的离子释放、微观结构和生物特性
DOI：
10.1016/j.joen.2016.10.011
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发表时间：
2017-01-01
影响因子：
4.2
通讯作者：
Wei, Xi
中科院分区：
文献类型：
--
作者：
Tian, Jun;Zhang, Yuanhe;Wei, Xi
本研究评估了将新型硅酸钙纳米颗粒生物陶瓷iRoot BP Plus （Innovative Bioceramix, Vancouver, Canada）暴露在酸性环境中如何影响该材料中的离子释放，并改变mc3l3 - e1在该材料上的成骨前细胞活力和附着。将这些因素与相似条件下的prooroot MTA进行比较。方法：将各材料分别置于磷酸盐缓冲盐水（pH = 7.4）或丁酸（pH = 5.4）中处理5 d。利用电感耦合等离子体发射光谱法对两种材料中的微量金属元素及其释放离子进行了鉴定。用扫描电子显微镜和能量色散x射线光谱仪测定了丁酸处理后MTA和iRoot BP Plus的微观结构和元素组成。并比较丁酸处理后MC3T3-E1细胞在材料表面的活力和对材料的附着水平。结果：iRoot BP Plus的有毒金属元素含量低于MTA。在酸性条件下，两种材料表现出相似的离子释放能力，硅和钙离子的释放增加。暴露于酸性ph后，两种材料的微观结构都发生了实质性变化，包括磷灰石的形成减少。此外，将iRoot BP Plus和MTA暴露于酸性环境中，分别增加和降低了这些材料上MC3T3-E1细胞的活力。MC3T3-E1细胞附着在这两种材料上都没有受到酸性ph值的显著影响。结论：iRoot BP Plus似乎比prooroot MTA更适合在炎症酸性环境中应用。
Introduction: This study evaluated how exposing the novel calcium silicate nanoparticulate bioceramic iRoot BP Plus (Innovative Bioceramix, Vancouver, Canada) to an acidic environment affects ion release from this material and alters MC3T3-E1 preosteoblast viability on and attachment to this material. These factors were compared against those of ProRoot MTA under similar conditions. Methods: Each material was exposed to phosphate buffered saline (pH = 7.4) or butyric acid (pH = 5.4) for 5 days. Trace metal elements within the 2 materials and released ions were identified using inductively coupled plasma optical emission spectroscopy. The microstructures and elemental compositions of MTA and iRoot BP Plus after treatment with butyric acid were determined using scanning electron microscopy with an energy dispersive X-ray spectrometer. Furthermore, the viability of MC3T3-E1 cells on and their levels of attachment to the materials after the butyric acid treatment were compared. Results: iRoot BP Plus contained fewer toxic metal elements than MTA. Under acidic conditions, both materials displayed similar ion release abilities, with increased release of Si and Ca ions. Substantial changes in microstructure, including reduced apatite formation, were observed for both materials after exposure to acidic pH. Furthermore, exposing iRoot BP Plus and MTA to an acidic environment increased and decreased MC3T3-E1 cell viability on these materials, respectively. MC3T3-E1 cell attachment to both materials was not significantly affected by acidic pH. Conclusions: iRoot BP Plus seems more biologically appropriate for application in an inflamed acidic environment than ProRoot MTA.