Tributyltin exposure results in craniofacial cartilage defects in rockfish (Sebastiscus marmoratus) embryos.
Tributyltin exposure results in craniofacial cartilage defects in rockfish (Sebastiscus marmoratus) embryos.
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DOI：
10.1016/j.marenvres.2011.12.008
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发表时间：
2012-06
影响因子：
3.3
通讯作者：
Jiliang Zhang;Zhenghong Zuo;P. Sun;Hongwei Wang;Ang Yu;Chonggang Wang
中科院分区：
文献类型：
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作者：
Jiliang Zhang;Zhenghong Zuo;P. Sun;Hongwei Wang;Ang Yu;Chonggang Wang
三丁基锡(TBT)是一种普遍存在的海洋环境污染物，已被认为会导致鱼类胚胎的中轴性骨骼畸形。然而，TBT对鱼类头面部软骨发育的影响尚不清楚。本研究旨在探讨不同环境水平(0、0.1、1和10 ng的L−1As锡)对石斑鱼胚胎头面部软骨发育的影响。我们的研究表明，TBT暴露可导致颅面骨骼畸形，如颅面骨骼元素减少和下颌骨变短。暴露后的岩鱼胚胎中，视黄酸受体α、声学刺激物和增殖细胞核抗原的表达受到抑制，维生素D受体的表达增加。此外，TBT暴露后，细胞内Ca~(2+)-ATPase活性受到抑制。提示TBT可能干扰软骨细胞的增殖和分化，扰乱钙稳态，从而影响颅面骨发育。
Tributyltin (TBT) is a ubiquitous marine environmental contaminant, which has been known to cause axial skeletal deformities in fish embryos. However, the effects of TBT on the craniofacial cartilage development of fishes remain unclear. The present study was designed to investigate the effects of waterborne TBT at environmental levels (0, 0.1, 1, and 10 ng L−1as Sn) on craniofacial cartilage development in embryos of the rockfish (Sebastiscus marmoratus). Our study showed that TBT exposure induced craniofacial skeletal deformities, such as reduction of the craniofacial skeleton elements and a shorter lower jaw. The expressions of retinoic acid receptor α, sonic hedgehog, and proliferating cell nuclear antigen were depressed and the expressions of vitamin D receptor were increased in the rockfish embryos after TBT exposure. In addition, the activities of Ca2+-ATPase were inhibited after TBT exposure. These results suggested that TBT might perturb the proliferation and differentiation of chondrocytes, and disturb calcium homeostasis, thus disorganizing craniofacial skeletal development.