Detection and identification of a single DNA base molecule using surface-enhanced Raman scattering (SERS)
Detection and identification of a single DNA base molecule using surface-enhanced Raman scattering (SERS)
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DOI：
10.1103/physreve.57.r6281
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发表时间：
1998-06-01
影响因子：
2.4
通讯作者：
Feld, MS
中科院分区：
文献类型：
--
作者：
Kneipp, K;Kneipp, H;Feld, MS
在近红外（NIR）激发下，每个分子的非共振拉曼散射截面约为10（-16）cm（2），这与银团簇的表面增强拉曼散射（Sers）有关。增强被发现是独立的集群大小之间的100 nm和20 μ m。这些实验结果表明，胶体银簇上的MR Sers是用于单分子检测的优异技术，其适用于广泛的分子，包括“无色”生物分子，例如DNA测序中的核苷酸。作为一个例子，我们提出了一个单一的腺嘌呤分子的检测没有任何标记的基础上，其固有的表面增强拉曼散射。
Nonresonant Raman cross sections of similar to 10(-16) cm(2) per molecule are shown to be related to surface-enhanced Raman scattering (SERS) on colloidal silver clusters at near-infrared (NIR) excitation. The enhancement is found to be independent of cluster sizes between 100 nm and 20 mu m. These experimental findings demonstrate that MR SERS on colloidal silver clusters is an excellent technique for single molecule detection that is applicable for a broad range of molecules including ''colorless" biomolecules, for example nucleotides in DNA sequencing. As an example, we present the detection of a single adenine molecule without any labeling based on its intrinsic surface-enhanced Raman scattering.