Effect of the PPARG2 Pro12Ala polymorphism and clinical risk factors for diabetes mellitus on HbA1c in the Japanese general population.
Effect of the PPARG2 Pro12Ala polymorphism and clinical risk factors for diabetes mellitus on HbA1c in the Japanese general population.
复制标题
DOI：
10.2188/jea.je20120078
复制
发表时间：
2012
影响因子：
4.7
通讯作者：
Japan Multi-Institutional Collaborative Cohort (J-MICC) Study Group
中科院分区：
文献类型：
--
作者：
Hara M;Higaki Y;Taguchi N;Shinchi K;Morita E;Naito M;Hamajima N;Takashima N;Suzuki S;Nakamura A;Ohnaka K;Uemura H;Nishida H;Hosono S;Mikami H;Kubo M;Tanaka H;Japan Multi-Institutional Collaborative Cohort (J-MICC) Study Group
虽然过氧化物酶体增殖物激活受体-γ2（PPARG 2）Pro 12 Ala基因变异与糖尿病相关，但该多态性和已知临床危险因素与糖化血红蛋白（HbA 1c）的关联和相互作用仍知之甚少。我们研究了携带Ala等位基因是否与HbA 1c水平呈负相关，并检查了可能的相互作用。这项横断面分析使用了从1281名男性和1356名女性中收集的数据，年龄在40至69岁之间，他们完成了日本多机构协作队列研究的基线调查。PPARG 2基因多态性检测采用多重聚合酶链反应（PCR）为基础的入侵检测。多元线性回归和ANCOVA用于控制混杂变量（年龄，体重指数[BMI]，能量摄入，酒精，吸烟，体力活动和糖尿病家族史），并检查可能的相互作用。校正后，女性Ala等位基因与HbA 1c呈显著负相关，但男性则无此相关。年龄较大、BMI和糖尿病家族史与男性和女性的HbA 1c较高相关。当按PPARG 2基因型分层时，在Pro 12 Pro基因型受试者中观察到这些相关性，但在Ala等位基因携带者中未观察到。在女性中观察到基因型和BMI对HbA 1c的显著交互作用。年龄大、BMI和糖尿病家族史与正常高值HbA 1c（≥ 5.7%NGSP）显著相关，而PPARG 2多态性与此无关。虽然PPARG 2 Pro 12 Ala多态性可能会减弱已知危险因素与HbA 1c水平之间的关联，但与临床危险因素相比，在一般人群中，它对正常高值HbA 1c的影响较小。
Although the peroxisome proliferator-activated receptor-γ2 (PPARG2) Pro12Ala gene variant is associated with diabetes mellitus, the associations and interactions of this polymorphism and known clinical risk factors with glycated hemoglobin (HbA1c) remain poorly understood. We investigated if carrying the Ala allele was inversely associated with HbA1c level and examined possible interactions. This cross-sectional analysis used data collected from 1281 men and 1356 women aged 40 to 69 years who completed the baseline survey of the Japan Multi-Institutional Collaborative Cohort Study. PPARG2 polymorphism was determined by multiplex polymerase chain reaction (PCR)-based Invader assay. Multiple linear regression and ANCOVA were used to control for confounding variables (age, body mass index [BMI], energy intake, alcohol, smoking, physical activity, and family history of diabetes) and examine possible interactions. After adjustment, the Ala allele was significantly inversely associated with HbA1c in women but not in men. Older age, BMI, and family history of diabetes were associated with higher HbA1c in both sexes. When stratified by PPARG2 genotype, these associations were observed in subjects with the Pro12Pro genotype but not in Ala allele carriers. A significant interaction of genotype and BMI on HbA1c was observed in women. Older age, BMI, and family history of diabetes were significantly associated with high-normal HbA1c (≥5.7% NGSP), whereas PPARG2 polymorphism was not. Although PPARG2 Pro12Ala polymorphism might attenuate associations between known risk factors and HbA1c level, it had a small effect on high-normal HbA1c, as compared with clinical risk factors, in the general population.