Intramural multisite recording of transmembrane potential in the heart
Intramural multisite recording of transmembrane potential in the heart
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DOI：
10.1016/s0006-3495(01)75910-3
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发表时间：
2001-11-01
影响因子：
3.4
通讯作者：
Smaill, BH
中科院分区：
文献类型：
--
作者：
Hooks, DA;LeGrice, IJ;Smaill, BH
心脏表面跨膜电位的光学标测已广泛应用于正常和病理性心律和除颤的研究。在这些研究中，三维时空事件只能从二维表面电位图推断。我们提出了一种新的光学系统，使高保真跨膜电位的跨壁记录。由光纤构成的探针用于通过兔心脏的左心室壁从七个位置（每个位置相距1 mm）传递激发光并收集荧光。激发由水冷氩离子激光器的488 nm线提供。来自每个组织部位的电压敏感染料di-4-ANEPPS的荧光在600 nm处分裂，并成像到单独的光电二极管上，用于稍后的信号比率。光学器件和电子器件易于扩展以容纳多个光学探头。该系统被用来记录跨膜电位的第一个同时测量在一些网站通过完整的心脏壁。
Heart surface optical mapping of transmembrane potentials has been widely used in studies of normal and pathological heart rhythms and defibrillation. In these studies, three-dimensional spatio-temporal events can only be inferred from two-dimensional surface potential maps. We present a novel optical system that enables high fidelity transmural recording of transmembrane potentials. A probe constructed from optical fibers Is used to deliver excitation light and collect fluorescence from seven positions, each 1 mm apart, through the left ventricle wall of the rabbit heart. Excitation Is provided by the 488-nm line of a water-cooled argon-ion laser. The fluorescence of the voltage-sensitive dye di-4-ANEPPS from each tissue site is split at 600 nm and imaged onto separate photodiodes for later signal ratioing. The optics and electronics are easily expandable to accommodate multiple optical probes. The system is used to record the first simultaneous measurements of transmembrane potential at a number of sites through the intact heart wall.