Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy
Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy
复制标题
使用鸟枪法策略进行综合磷酸化蛋白质组分析的前景
DOI：
10.1021/ac201833j
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发表时间：
2011-11-01
影响因子：
7.4
通讯作者：
Zou, Hanfa
中科院分区：
文献类型：
--
作者：
Wang, Fangjun;Song, Chunxia;Zou, Hanfa
蛋白质磷酸化是一种普遍存在的翻译后修饰，调节几乎所有的细胞过程。由于分析物的高动态范围和低丰度性质，蛋白质磷酸化的分析具有挑战性。基于鸟枪的蛋白质组学已经成为一个非常有用的平台，以实现一个全面的磷酸化蛋白质组分析相当深入。近几年来，随着相关技术的飞速发展，磷酸化的大规模分析也取得了沿着的突破。有效的富集材料，精细的分析工作流程，新型强大的质谱仪和复杂的生物信息学工具的组合大大提高了性能。综合磷酸化蛋白质组分析。在这个角度来看，我们简要地回顾了富集材料，预分馏工作流程，不同的质谱裂解模式，以及磷酸化蛋白质组鉴定和定量的软件工具的最新技术发展。然后，我们描述了当前的挑战和未来的综合磷酸化蛋白质组分析的潜在方向。我们还提供了如何进一步提高相关分析方法和技术的性能的观点。
Protein phosphorylation is a ubiquitous post-translational modification that regulates almost all cellular processes. The analysis, of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of the analyte. Shotgun based proteomics has emerged as a very useful platform to achieve a comprehensive phosphoproteome analysis in considerable depth. In the past few years, significant breakthroughs on the large scale phosphorylation analysis have been witnessed along with the great development of related technologies. The combination of effective enrichment materials, refined analysis workflows, new type of powerful mass spectrometers, and sophisticated bioinformatic tools greatly boost the performance of. comprehensive phosphoproteome analysis. In this Perspective, we briefly reviewed recent technological developments on the enrichment materials, prefractionation workflows, and different Mass spectrometry fragmentation modes as well as software tools for phosphoproteome identification and quantification. Then, we described the current challenges and potential directions for the future of comprehensive phosphoproteome analysis. We also provide perspectives on how to further improve the performance of related analysis methods and technologies.