Molecular evolution and phylogeny of dengue-4 viruses
Molecular evolution and phylogeny of dengue-4 viruses
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DOI：
10.1099/0022-1317-78-9-2279
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发表时间：
1997-09-01
影响因子：
3.8
通讯作者：
Trent, DW
中科院分区：
文献类型：
--
作者：
Lanciotti, RS;Gubler, DJ;Trent, DW
测定了19种不同地理和时间的登革4型病毒的囊膜蛋白基因的核苷酸序列。核酸序列比较表明，登革4型病毒之间的同源性大于92%，推导的氨基酸序列之间的相似性在96 ~ 100%之间，在大多数情况下，来自相似地理区域的病毒之间存在相同的氨基酸替换。核酸序列的比对和简约性分析生成系统发育树，表明DEN-4病毒的进化具有地理独立性。DEN-4病毒分为两种遗传上不同的亚型（基因型）。基因型1包含来自菲律宾、泰国和斯里兰卡的病毒;基因型2包含来自印度尼西亚、塔希提岛、加勒比群岛（波多黎各、多米尼加）以及中美洲和南美洲的病毒。
Nucleotide sequences of the envelope protein genes of 19 geographically and temporally distinct dengue (DEN)-4 viruses were determined. Nucleic acid sequence comparison revealed that the identity among the DEN-4 viruses was greater than 92%, Similarity among deduced amino acids was between 96 and 100%; in most cases identical amino acid substitutions occurred among viruses from similar geographical regions. Alignment of nucleic acid sequences followed by parsimony analysis generated phylogenetic trees, which indicated that geographically independent evolution of DEN-4 viruses had occurred. DEN-4 viruses were separated into two genetically distinct subtypes (genotypes). Genotype-1 contains viruses from the Philippines, Thailand and Sri Lanka; genotype-2 consists of viruses from Indonesia, Tahiti, the Caribbean Islands (Puerto Rico, Dominica) and Central and South America.