Capturing the Interactome of Newly Transcribed RNA
Capturing the Interactome of Newly Transcribed RNA
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DOI：
10.1038/nmeth.4595
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
C
中科院分区：
文献类型：
--
作者：
Xichen Bao;Xiangpeng Guo;Menghui Yin;Muqdas Tariq;Yiwei Lai;Shahzina Kanwal;Jiajian Zhou;Na Li;Yuan Lv;Carlos Pulido-Quetglas;Xiwei Wang;Lu Ji;Muhammad J. Khan;Xihua Zhu;Zhiwei Luo;Changwei Shao;Do-Hwan Lim;Xiao Liu;Nan Li;Wei Wang;Minghui He;Yu-Lin Liu;C
Application of synthetic nucleoside analogs to capture nascent RNAs has unveiledkey features of gene transcription. Whether similar approaches can be adapted to ..isolate the RNA-bound proteome was, however, unexplored. Here, we present a methodology (capture of the newly transcribed RNA Interactome using ClicK chemistry; RICK) ..based on the labeling of newly transcribed RNAs with 5-ethynyluridine, which systematically captures proteins bound to a wide range of RNAs including nascent RNAs and ..traditionally neglected non-polyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for non-..polyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and also expanded the known RNA-bound proteome of mouse embryonic stem cells. ..Thanks to its versatility, RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.
