Induction ofhemocyte apoptosis byOvomermis sinensis: Implications for host immune suppression
Induction ofhemocyte apoptosis byOvomermis sinensis: Implications for host immune suppression
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DOI：
10.1016/j.jip.2018.10.011
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Guoxiu Wang
中科院分区：
文献类型：
--
作者：
Zhenlong Jiao;Guigui Wena;...;Guoxiu Wang
The entomopathogenic nematode, Ovomermis sinensis, is a parasite of some common lepidopteran pests. O. sinensis is able to overcome the immune system of its hosts and eventually kill the hosts when it emerges. We provide insight into how the mermithid nematode overcomes the immune response of the host Helicoverpa armigera. Our results indicate that O. sinensis actively inhibits host immune responses as evidenced by hemocyte nodulation, spreading behavior, lysozyme activity and melanization. However, O. sinensis did not inhibit host immuneresponsesthroughimmunegeneactivation.Moreover,theresearchontheimmunedepressivestrategies of O. sinensis revealed that the parasite did not inhibit host effector molecules, but did reduce the number of hemocytes. Flow cytometry analysis revealed that the host hemocytes were apoptotic within 24h, and no hemocytes were present after 72h. In addition, our in vivo and in vitro studies showed that the survival rate of O. sinensis was increased when hemocyte proliferation was inhibited. Our findings suggest that O. sinensis inhibited host immune responses by inducing apoptosis of host hemocytes..
