TRAPPING, DETECTION, AND MASS MEASUREMENT OF INDIVIDUAL IONS IN A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETER
TRAPPING, DETECTION, AND MASS MEASUREMENT OF INDIVIDUAL IONS IN A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETER
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DOI：
10.1021/ja00096a046
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发表时间：
1994-08-24
影响因子：
15
通讯作者：
SMITH, RD
中科院分区：
文献类型：
--
作者：
BRUCE, JE;CHENG, X;SMITH, RD
傅里叶变换离子回旋共振（FTICR）质谱仪已被用来捕获个别多电荷离子的几个高分子量聚合物，包括聚（环氧乙烷），聚（苯乙烯磺酸钠），和蛋白质牛血清白蛋白。这些离子的检测是用FTICR特有的非破坏性方法进行的，该方法还允许在几个小时内重新测量相同的离子或离子群。为了确定单个离子的电荷状态（从而确定其质量），在观察由电荷交换反应或已知质量的物质的加合产生的逐步MIT位移的基础上，开发了一种新的方案。一种新的技术，用于单个离子的质量测定已成为可能，在时域采集期间的回旋频率偏移的观察。这种时间分辨离子相关（TRIC）技术允许反应物和产物离子与置信度相关，并为同时研究中等数量的离子提供了基础。在这项工作中，一系列的观察有关的检测和测量的个别离子，是通过利用TRIC技术进行个别离子的质量测定的例子。结果表明，聚（乙二醇）（PEG）的单个离子的测量超过5 MDa与超过2500净电荷和测量的离子小白蛋白（66 kDa）与少至30个电荷。其他结果表明，可以观察到单个离子的意外行为，这在大离子群中并不明显。
A Fourier transform ion cyclotron resonance (FTICR) mass spectrometer has been used to trap individual multiply charged ions of several high molecular weight polymers, including poly(ethylene oxide), sodium poly(styrene sulfonate), and the protein bovine serum albumin. Detection of these ions is performed with the nondestructive method distinctive of FTICR, which also allows remeasurement of the same ion or ion population over several hours. For the determination of the charge states (and hence the masses) of individual ions, a new scheme was developed on the basis of the observation of the stepwise mit shifts that result from charge exchange reactions or adduction of a substance of known mass. A novel technique for mass determination of individual ions has been made possible with the observation of cyclotron frequency shifts during the time-domain acquisition period. This time-resolved ion correlation (TRIC) technique allows reactant and product ions to be correlated with confidence and provides the basis for simultaneously studying a moderate number of ions. In this work, a range of observations related to the detection and measurement of Individual ions is presented, as are examples of mass determinations of individual ions performed by utilizing the TRIC technique. Results are presented that show the measurement of poly(ethylene glycol) (PEG) individual ions of more than 5 MDa with more than 2500 net charges and measurement of ions as small as albumin (66 kDa) with as few as 30 charges. Other results illustrate that unexpected behavior can be observed for individual ions that would not be apparent in large ion populations.