A novel centrifugal impeller bioreactor. II. Oxygen transfer and power consumption
A novel centrifugal impeller bioreactor. II. Oxygen transfer and power consumption
复制标题
DOI：
10.1002/(sici)1097-0290(19960905)51:5
复制
发表时间：
2000-03
影响因子：
3.8
通讯作者：
Si‐Jing Wang;J. Zhong
中科院分区：
文献类型：
--
作者：
Si‐Jing Wang;J. Zhong
在新设计的离心叶轮生物反应器（5-L）中研究了叶轮配置、通气速率和搅拌速度对氧传递系数 KLa 的影响。新型生物反应器中的氧转移速率也与具有相当尺寸的细胞提升生物反应器中的氧转移速率进行了比较。在搅拌速度超过 200 rpm 时，细胞提升叶轮产生的表面氧转移率比离心叶轮高得多。这个结果与我们的观察结果非常一致，即细胞升力叶轮产生了更高的不利湍流。此外，使用颗粒化琼脂颗粒作为假植物细胞的实验表明，随着琼脂颗粒浓度的增加，KLa值稳步下降，并且离心叶轮仍然表现出比细胞升力更大的KLa，直至假细胞浓度达到19.5 g干重（DW）/L（在150 rpm和0.20 vvm下）或22.3 g DW/L（在200 rpm和0.20 vvm下） vvm）。此外，成功获得了离心式叶轮和单元升力式叶轮的功率数与叶轮雷诺数之间的相关性，可用于预测每个叶轮所需的功率输入。从所获得的结果来看，离心叶轮生物反应器预计在剪切敏感生物系统中具有巨大的应用潜力。
The effects of the impeller configuration, aeration rate, and agitation speed on oxygen transfer coefficient KLa were studied in a newly designed centrifugal impeller bioreactor (5‐L). The oxygen transfer rates in the novel bioreactor were also compared with those in a cell‐lift bioreactor with comparable dimensions. The cell‐lift impeller produced much higher surface oxygen transfer rates than the centrifugal one at an agitation speed over 200 rpm. This result was in good agreement with our observation that the cell‐lift impeller produced much higher unfavorable turbulence. In addition, the experiments using granulated agar particles as pseudo plant cells indicated that the KLa value decreased steadily with an increase in agar particle concentration, and the centrifugal impeller still demonstrated a larger KLa than the cell lift up to a high pseudo cell concentration of 19.5 g dry weight (DW)/L (under 150 rpm and 0.20 vvm) or 22.3 g DW/L (under 200 rpm and 0.20 vvm). Furthermore, the correlation between power number and impeller Reynolds number for both the centrifugal and the cell‐lift impellers was successfully obtained, which could be used for predicting the power input required by each impeller. From the results obtained, the centrifugal impeller bioreactor is expected to have great potential in its application to shear‐sensitive biological systems.