THESEUS1 positively modulates plant defense responses against Botrytis cinerea through GUANINE EXCHANGE FACTOR4 signaling.
THESEUS1 positively modulates plant defense responses against Botrytis cinerea through GUANINE EXCHANGE FACTOR4 signaling.
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DOI：
10.1111/jipb.12565
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发表时间：
2017-06
影响因子：
11.4
通讯作者：
Shaofeng Qu;Xi Zhang;Yutong Song;Jinxing Lin;Xiaoyi Shan
中科院分区：
文献类型：
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作者：
Shaofeng Qu;Xi Zhang;Yutong Song;Jinxing Lin;Xiaoyi Shan
植物细胞壁是感受病原体攻击和激活促进植物免疫反应的信号传导途径的重要界面。THESEUS 1（THE 1）作为细胞壁完整性的传感器，在植物生长过程中控制细胞伸长。然而，没有具体的作用THE 1在植物防御反应的报道。在这里，我们发现THE 1与鸟嘌呤交换因子4（GEF 4）相互作用，这两种蛋白质在植物对坏死型真菌灰葡萄孢的抗性中起调节作用。遗传分析表明，THE 1和GEF 4通过相同的遗传途径介导植物的防御反应。此外，使用转录组分析，我们确定了各种基因（如防御相关，次级代谢相关和转录因子基因），这些基因可能是THE 1-GEF 4信号通路的下游靶点。我们的研究结果表明，THE 1作为GEF 4信号传导的上游调节因子，积极调节对B的防御反应。cinerea in Arabidopsis.
The plant cell wall is an important interface for sensing pathogen attack and activating signaling pathways that promote plant immune responses. THESEUS1 (THE1) acts as a sensor of cell wall integrity that controls cell elongation during plant growth. However, no specific role for THE1 in plant defense responses has been reported. Here, we found that THE1 interacts with GUANINE EXCHANGE FACTOR4 (GEF4) and that both proteins play regulatory roles in plant resistance to the necrotrophic fungus Botrytis cinerea. Genetic analysis showed that THE1 and GEF4 function in the same genetic pathway to mediate plant defense responses. In addition, using transcriptome analysis, we identified various genes (such as defense-related, secondary metabolite-related, and transcription factor genes) that are likely downstream targets in the THE1-GEF4 signaling pathway. Our results suggest that THE1 functions as an upstream regulator of GEF4 signaling to positively regulate defense responses against B. cinerea in Arabidopsis.