Behavioral and molecular responses to electroconvulsive shock differ between genetic and environmental rat models of depression
Behavioral and molecular responses to electroconvulsive shock differ between genetic and environmental rat models of depression
复制标题
遗传性和环境性抑郁症大鼠模型对电休克的行为和分子反应不同
DOI：
10.1016/j.psychres.2014.12.068
复制
发表时间：
2015-04-01
影响因子：
11.3
通讯作者：
Liu, Yuanyuan
中科院分区：
文献类型：
--
作者：
Luo, Jie;Min, Su;Liu, Yuanyuan
抑郁的原因在个体对抗抑郁药物治疗的不同反应中起着作用，抗抑郁药物治疗需要进步。我们在具有不同(遗传或环境)抑郁原因的大鼠模型中，研究了一种高效的抗抑郁药物治疗--电休克治疗(ECT)背后的机制和对该治疗的反应。Wistar京都(WKY)大鼠和慢性不可预测轻度应激(CUMS)处理的Wistar大鼠分别作为抑郁症的遗传性和环境性大鼠模型。大鼠接受电惊厥休克(ECS，类似于ECT)或假ECS。我们进行了蔗糖偏好测试、开场测试和Morris水迷宫来评估行为。尼氏染色检测海马神经元数量。用双抗体夹心法或免疫印迹法检测大鼠海马区BDNF、CREB和p-CREB蛋白的表达。主要结果表明，ECS对WKY大鼠的记忆有损害作用，但对CUMS大鼠的记忆有改善作用。它只提高了CUMS大鼠的海马区BDNF和CREB蛋白水平，而改善了两只大鼠的抑郁行为和海马区p-CREB蛋白水平，对CUMS大鼠的调节更为有效。ECS对两组大鼠海马神经元数量无明显影响。这些结果表明，ECS对抑郁大鼠的海马区BDNF和CREB(及其磷酸化)有上调作用，且环境模型的反应更好。(C)2015爱思唯尔爱尔兰有限公司。保留所有权利。
Depression's causes play a role in individuals' different responses to antidepressant treatments, which require advancements. We investigated the mechanisms behind and responses to a highly effective antidepressant treatment, electroconvulsive therapy (ECT), in rat models with different (genetic or environmental) depression causes. Wistar Kyoto (WKY) rats and Wistar rats treated with chronic unpredictable mild stresses (CUMS) were used as genetic and environmental rat models of depression, respectively. The rats underwent electroconvulsive shock (ECS, the animal analog of ECT) or sham ECS. We performed a sucrose preference test, open field test, and Morris water maze to assess behavior. Hippocampal neuron numbers were measured with Nissl stain. Hippocampal BDNF, CREB, and p-CREB proteins were assayed with ELISA or western blotting. The main results showed that ECS impaired WKY rats' memories but improved CUMS rats' memories. It elevated hippocampal BDNF and CREB proteins only in CUMS rats, while it improved depressive behavior and hippocampal p-CREB protein levels in both rats, with more effective regulations in the CUMS rats. ECS did not change the hippocampal neuron number in both rats. These findings suggest that ECS exerted up-regulating effects on hippocampal BDNF and CREB (and its phosphorylation) in depressed rats, and the environmental model responded better. (C) 2015 Elsevier Ireland Ltd. All rights reserved.