A naturally occurring human/hepatitis E recombinant virus predominates in serum but not in faeces of a chronic hepatitis E patient and has a growth advantage in cell culture
A naturally occurring human/hepatitis E recombinant virus predominates in serum but not in faeces of a chronic hepatitis E patient and has a growth advantage in cell culture
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DOI：
10.1099/vir.0.037259-0
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发表时间：
2012-03-01
影响因子：
3.8
通讯作者：
Emerson, S. U.
中科院分区：
文献类型：
--
作者：
Nguyen, H. T.;Torian, U.;Emerson, S. U.
戊型肝炎病毒是急性戊型肝炎的病原体，急性戊型肝炎是一种在发展中国家流行的自限性疾病。对慢性感染患者的病毒基因组RNA的分子分析证实了最近的发现，即慢性感染与病毒准种的广泛多样性相关：该美国患者的某些病毒基因组的高变区含有大的连续缺失，粪便和血清中的一小部分基因组获得了编码与病毒非融合的S19核糖体蛋白的39个氨基酸的哺乳动物序列，结构蛋白在培养细胞中病毒传代期间选择具有该插入物的基因组成为优势种，表明插入序列促进病毒生长。结果表明，戊型肝炎病毒在长期感染期间会发生显著突变，并表明在出现具有不可预测特性的新变体之前预防或治愈慢性感染可能很重要。
Hepatitis E virus is the aetiological agent of acute hepatitis E, a self-limiting disease prevalent in developing countries. Molecular analysis of viral genomic RNA from a chronically infected patient confirmed the recent discovery that chronic infection correlated with extensive diversification of the virus quasispecies: the hypervariable region of some virus genomes in this USA patient contained large continuous deletions and a minor proportion of genomes in faeces and serum had acquired a mammalian sequence that encoded 39 aa of S19 ribosomal protein fused to the virus non-structural protein. Genomes with this insert were selected during virus passage in cultured cells to become the predominant species, suggesting that the inserted sequence promoted virus growth. The results demonstrated that hepatitis E virus can mutate dramatically during a prolonged infection and suggests it may be important to prevent or cure chronic infections before new variants with unpredictable properties arise.