Novel approaches to map small molecule-target interactions
Novel approaches to map small molecule-target interactions
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DOI：
10.1016/j.bmc.2016.05.020
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发表时间：
2016-08-01
影响因子：
3.5
通讯作者：
Ziegler, Slava
中科院分区：
文献类型：
--
作者：
Kapoor, Shobhna;Waldmann, Herbert;Ziegler, Slava
对蛋白质功能或特定细胞过程的小分子干扰剂的探索是化学生物学和药物研究的核心。生物活性化合物需要在生物系统中调节的蛋白质或过程的背景下进行广泛表征，以揭示和确认其作用模式。这个工作流程中的一个关键步骤是鉴定这些小分子的分子靶点，这是缺乏通用方法的。在这里，我们总结了最近开发的方法，目标识别刺激组学技术和化学和生物信息学分析的进步。(C)2016爱思唯尔有限公司版权所有
The quest for small molecule perturbators of protein function or a given cellular process lies at the heart of chemical biology and pharmaceutical research. Bioactive compounds need to be extensively characterized in the context of the modulated protein(s) or process(es) in living systems to unravel and confirm their mode of action. A crucial step in this workflow is the identification of the molecular targets for these small molecules, for which a generic methodology is lacking. Herein we summarize recently developed approaches for target identification spurred by advances in omics techniques and chemo- and bioinformatics analysis. (C) 2016 Elsevier Ltd. All rights reserved.