Correlation between intima-to-media ratio, apolipoprotein B-100, myeloperoxidase, and hypochlorite-oxidized proteins in human atherosclerosis
Correlation between intima-to-media ratio, apolipoprotein B-100, myeloperoxidase, and hypochlorite-oxidized proteins in human atherosclerosis
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DOI：
10.1016/s0891-5849(01)00717-1
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发表时间：
2001-11-15
影响因子：
7.4
通讯作者：
Stocker, R
中科院分区：
文献类型：
--
作者：
Hazell, LJ;Baernthaler, G;Stocker, R
低密度脂蛋白（LDL）的氧化修饰被认为有助于动脉粥样硬化形成，有证据表明，髓过氧化物酶（MPO）衍生的氧化剂有助于这种氧化损伤。使用人髂动脉，我们研究了内膜-中膜（I/M）比值所示病变分期与载脂蛋白B-100（apo B，LDL标记物）、MPO和次氯酸盐（HOCI）氧化蛋白在内膜、中膜和外膜中的存在之间的关系。在病变血管中观察到apoB、MPO和HOCI氧化蛋白的染色多于健康血管。在同一病变血管中，与健康节段相比，病变节段对三个参数的染色也更多，突出显示了血管单个横截面内可能发生的变异性。然而，在I/M比> 1.8的动脉段中，I/M比与apoB、MPO和HOCI-氧化蛋白在不同动脉段中的阳性染色之间显著正相关。此外，内膜apoB、MPO和HOCI氧化蛋白染色的总体程度随着I/M比的增加而增加。此外，apoB染色的程度更大，出现在相对较低的I/M比比MPO和HOCI氧化蛋白。我们的研究结果支持评估的所有三个参数的动脉粥样硬化的贡献，虽然MPO和HOCI氧化蛋白似乎参与疾病过程中的后期比载脂蛋白B。(C)2001 Elsevier Science Inc.
The oxidative modification of low-density lipoprotein (LDL) is thought to contribute to atherogenesis, and there is evidence that oxidants derived from myeloperoxidase (MPO) contribute to such oxidative damage. Using human iliac arteries we investigated the relationship between lesion stage indicated by the intima-to-media (I/M) ratio and the presence of apolipoprotein B-100 (apoB, a marker for LDL), MPO, and hypochlorite (HOCI)-oxidized proteins identified by immunohistochemistry in the intima, media, and adventitia. More staining for apoB, MPO, and HOCIoxidized proteins was observed in diseased than healthy vessels. Diseased segments also stained more for the three parameters than healthy segments in the same diseased vessel, highlighting the variability that can occur within a single cross-section of a vessel. However, significant positive correlation between I/M ratio and positive staining for apoB, MPO, and HOCI-oxidized proteins in different segments of individual arteries were apparent in segments with an I/M ratio of > 1.8. Also, the overall extent of intimal staining for apoB, MPO, and HOCI-oxidized proteins increased with increasing, I/M ratio. In addition, the extent of apoB staining was greater and appeared at comparatively lower I/M ratios than that of MPO and HOCI-oxidized proteins. Our results support a contribution to atherogenesis of all three parameters assessed, although MPO and HOCI-oxidized proteins appear to participate in the disease process at a later stage than apoB. (C) 2001 Elsevier Science Inc.