Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study
Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study
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DOI：
10.1016/j.chroma.2010.12.118
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发表时间：
2011-09-09
影响因子：
4.1
通讯作者：
Sutherland, Ian
中科院分区：
文献类型：
--
作者：
Zhang, Min;Ignatova, Svetlana;Sutherland, Ian
本文提出了一种利用逆流色谱技术开发绿色工艺的策略。该策略从溶剂系统的选择开始，然后通过优化操作参数从分析过程线性放大到制备过程。采用多次进样法进行两级分离，第一级溶剂系统为己烷-二氯甲烷-甲醇-水（4：0.75：4：1），第二级溶剂系统为己烷-乙醇-水（4：2：2）。从2.1g粗提物中纯化得到1918 mg丹参酮IIA，纯度为97%，回收率为34.4%;从2.1g粗提物中分离得到276.7mg隐丹参酮，纯度为95%，回收率为31.8%。开发了用于监测逆流色谱工艺的工艺参数（通量、效率、环境风险因子和一般工艺评价）和目标的质量因子（质量强度、分离质量效率和绿色度）。(C)2011 Elsevier B. V.保留所有权利。
A strategy for the development of a green process using counter-current chromatography technology is presented in this paper. The strategy began with solvent system selection, followed by linear scale-up from an analytical to a preparative process with optimized operating parameters. A two-stage separation using a multi-injection method was performed with a solvent system of hexane-dichloromethane-methanol-water (4:0.75:4:1) for the 1st stage and a hexane-ethanol-water (4:2:2) for the 2nd stage. A 1918 mg of tanshinone IIA was purified, with a 97% purity and 34.4% recovery and a 276.7 mg of cryptotanshinone was separated, with a 95% purity and 31.8% recovery from 2.1 g of crude extract. Process parameters (throughput, efficiency, environmental risk factor and general process evaluation) and mass factors (mass intensity, separation mass efficiency and greenness) of a target were developed for monitoring of the counter-current chromatography process. (C) 2011 Elsevier B.V. All rights reserved.