Different roles of crayfish hemocytes in the uptake of foreign particles
Different roles of crayfish hemocytes in the uptake of foreign particles
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小龙虾血细胞在吸收异物中的不同作用
DOI：
10.1016/j.fsi.2018.03.029
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发表时间：
2018-06-01
影响因子：
4.7
通讯作者：
Yang, Feng
中科院分区：
文献类型：
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作者：
Li, Fang;Chang, Xuefeng;Yang, Feng
已知甲壳动物血细胞通过吞噬作用清除入侵的病原体。本研究探讨了红螯螯虾的半颗粒细胞（SGCs）和颗粒细胞（GCs）如何参与这一过程。通过给动物注射过量的荧光微球（FM），我们发现只有一小部分循环血细胞是吞噬细胞，它们以大小依赖的方式摄取FM。0.2 μ m的FMs几乎完全被SGCs内化，而GCs和SGCs都有助于2 μ m FMs的吸收。进一步分析注射FM混合物动物的血细胞表明，存在一个SGCs亚群，特异性摄取0.2 μ m FM。尺寸依赖的方式也适用于生物颗粒。大肠杆菌被SGCs和GC内化，而白色斑点综合征病毒（WSSV）主要被SGCs摄入。然而，细菌细胞被血细胞迅速从循环中清除，而WSSV病毒粒子逐渐内化并在细胞中保留相对较长的时间。这些发现提供了螯虾吞噬血细胞的基本信息，以及它们如何对不同的外来颗粒作出反应。
Crustacean hemocytes are known to remove invading pathogens by phagocytosis. In this study, we investigated how the semigranular cells (SGCs) and granular cells (GCs) of crayfish Cherax quadricarinatus participated in this process. By injecting the animals with excessive amounts of fluorescent microspheres (FMs), we showed that only a small portion of the circulating hemocytes were phagocytic cells, and they took up FMs in a size-dependent manner. The 0.2 mu m FMs were internalized almost entirely by SGCs, while GCs and SGCs both contributed to the uptake of 2 mu m FMs. Further analysis of the hemocytes from the animals injected with a mixture of FMs suggested that there were a subpopulation of SGCs specifically ingesting 0.2 mu m FMs. The size-dependent manner was also applied to biological particles. Escherichia coli was internalized by both SGCs and GCs, whereas white spot syndrome virus (WSSV) was mostly ingested by SGCs. However, the bacterial cells were rapidly taken and cleared from the circulation by the hemocytes, while the WSSV virions were gradually internalized and remained in the cells for a relatively longer period of time. These findings provide basic information of the phagocytic hemocytes of crayfish and how they respond to different foreign particles.