Expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma: association with features of differentiation
Expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma: association with features of differentiation
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DOI：
10.1136/jclinpath-2011-200400
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发表时间：
2012-05-01
影响因子：
3.4
通讯作者：
Sawada, N.
中科院分区：
文献类型：
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作者：
Soini, Y.;Takasawa, A.;Sawada, N.
目的 本研究旨在评估 Claudins 7 和 18 在胰腺导管腺癌中的表达。 方法和结果 测试材料包括 111 例手术样本和 47 例额外活检样本，其中包括 26 例胰腺炎、3 例胰腺上皮内瘤变和 18 例导管腺癌。用密蛋白 7 和 18 的抗体对样品进行染色，并分析膜和细胞质的表达。膜结合紧密蛋白 7 和 18 的表达分别在 105 例中的 62 例 (59%) 和 111 例中的 78 例 (70%) 中检测到。膜结合紧密蛋白 7 和 18 与大或中型肿瘤性导管相关（分别为 p=0.01、p=0.002）。高分化胰腺腺癌显示更多膜结合密蛋白 7 或 18 免疫阳性病例（分别为 p=0.003、p=0.03）。研究的所有胰腺上皮内肿瘤均表达膜结合紧密蛋白 18。膜结合紧密蛋白 7 或 18 阳性与生存无关 (p=0.17, p=0.98)。在活检病例中，膜结合的claudin 18对肿瘤标志物具有100%的特异性和51%的敏感性。结论 Claudin 7和18的表达与肿瘤细胞的腺体大小有关，尤其见于中、大导管肿瘤和分化良好的肿瘤。膜结合紧密蛋白 18（当存在时）是诊断胰腺癌的有用标记物。 Claudins 7 和 18 与患者存活或肿瘤扩散无关。
Aim This study was undertaken to evaluate the expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma.Methods and results Material tested included 111 operated samples and 47 additional biopsy samples consisting of 26 cases of pancreatitis, 3 cases of pancreatic intraepithelial neoplasia and 18 ductal adenocarcinomas. Samples were stained with antibodies to claudins 7 and 18 and analysed for membranous and cytoplasmic expression. Membrane bound claudin 7 and 18 expression was detected in 62 of 105 (59%) and 78 of 111 (70%) cases, respectively. Membrane bound claudin 7 and 18 were associated with large or intermediate neoplastic ducts (p=0.01, p=0.002, respectively). Well differentiated pancreatic adenocarcinomas displayed more cases with membrane bound claudin 7 or 18 immunopositivity (p=0.003, p=0.03, respectively). All pancreatic intraepithelial neoplasias studied expressed membrane bound claudin 18. Membrane bound claudin 7 or 18 positivity was not associated with survival (p=0.17, p=0.98). In the biopsy cases membrane bound claudin 18 had 100% specificity and 51% sensitivity for a tumour marker.Conclusion Claudin 7 and 18 expression is related to gland size of neoplastic cells and is especially found in tumours with intermediate and large ducts and well differentiated tumours. Membrane bound claudin 18, when present, is a useful marker for diagnosis of pancreatic cancer. Claudins 7 and 18 were not associated with patient survival or spread of tumours.