Surveillance of chronic wasting disease in sika deer, Cervus nippon, from Tokachi district in Hokkaido
Surveillance of chronic wasting disease in sika deer, Cervus nippon, from Tokachi district in Hokkaido
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DOI：
10.1292/jvms.67.349
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发表时间：
2005-03-01
影响因子：
1.2
通讯作者：
Ishiguro, N
中科院分区：
文献类型：
--
作者：
Kataoka, N;Nishimura, M;Ishiguro, N
对北海道十胜地区136只被猎杀的西卡（Cervus nippon）的组织样品进行了Western印迹分析，以监测慢性消耗病（CWD）。在所有样本中均未检测到与慢性消耗病相关的朊病毒蛋白（PrPSc）。为了评估西卡鹿PrP基因的氨基酸多态性，对PrP基因进行核苷酸测序。检测到的唯一氨基酸多态性是核苷酸位置63、225和408处的3个沉默突变。这些结果表明，西卡鹿在十胜区的遗传同质，并没有感染慢性消耗病。
Surveillance of chronic wasting disease (CWD) was conducted by performing Western blot analysis of tissue samples from 136 sika deer (Cervus nippon) killed by hunters in the Tokachi district of Hokkaido Island. No prion protein (PrPSc) associated with CWD was detected in any of the samples. To assess amino acid polymorphisms of the sika deer PrP gene, nucleotide sequencing of the PrP gene was performed. The only amino acid polymorphisms detected were 3 silent mutations at nucleotide positions 63, 225 and 408. These results suggest that sika deer in the Tokachi district are genetically homogeneous, and are not infected with CWD.