Specific aspects of flow cytometric analysis of cells from the lung
Specific aspects of flow cytometric analysis of cells from the lung
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DOI：
10.1016/j.etp.2006.02.009
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发表时间：
2006-06-01
影响因子：
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通讯作者：
Garn, Holger
中科院分区：
文献类型：
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作者：
Garn, Holger
流式细胞术是一种分析细胞功能的强大方法，因此已应用于包括呼吸研究在内的生物医学研究的各个领域。当研究来自肺的细胞时，必须考虑一些特定的方面，包括来自不同肺室的细胞的制备和分析以及由于肺泡巨噬细胞的高自发荧光引起的问题。本简短的评论讨论了这些问题，此外，给出了一个例子，最近开发的应用流式细胞术分析在肺部研究领域，允许居民和新浸润的肺泡巨噬细胞之间的分化。(c)2006年Elsevier GmbH。All rights reserved.
Flow cytometry represents a powerful method for the analysis of cell functions and has therefore been applied in all fields of biomedical research including respiratory research. Some specific aspects have to be considered when investigating cells from the lung that include preparation and analysis of cells from different lung compartments and problems evoking due to high autofluorescence of alveolar macrophages. The present short review discusses these issues and, in addition, gives an example for a recently developed application of flow cytometric analysis in the field of lung research that allows the differentiation between resident and newly infiltrated alveolar macrophages. (c) 2006 Elsevier GmbH. All rights reserved.