Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm
Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm
复制标题
DOI：
10.1007/s00299-008-0625-7
复制
发表时间：
2009-02-01
影响因子：
6.2
通讯作者：
Toriyama, Kinya
中科院分区：
文献类型：
--
作者：
Itabashi, Etsuko;Kazama, Tomohiko;Toriyama, Kinya
LD型细胞质雄性不育（CMS）水稻具有“铅稻”的细胞质，其育性由核育性恢复基因Rf1恢复。 Rf1 在具有“Chinsurah Boro II”细胞质的 BT-CMS 中促进 CMS 相关线粒体 RNA atp6-orf79 的加工，该线粒体 RNA 由 atp6 和 orf79 组成。在这项研究中，我们发现LD细胞质中含有atp6下游orf79的序列变体。 Northern印迹分析表明，LD细胞质的atp6-orf79 RNA是共转录的，并且在Rf1存在的情况下以与BT细胞质中相同的方式进行加工。 Western blot分析显示ORF79肽在LD-CMS系中不积累，而ORF79在BT-CMS系中积累并被Rf1减少。这些结果表明ORF79的积累不是LD-细胞质中CMS的原因，并且LD-CMS中雄性不育诱导/生育性恢复的机制不同于BT-CMS。
Rice with LD-type cytoplasmic male sterility (CMS) possesses the cytoplasm of 'Lead Rice' and its fertility is recovered by a nuclear fertility restorer gene Rf1. Rf1 promotes processing of a CMS-associated mitochondrial RNA of atp6-orf79, which consists of atp6 and orf79, in BT-CMS with the cytoplasm of 'Chinsurah Boro II'. In this study, we found that LD-cytoplasm contained a sequence variant of orf79 downstream of atp6. Northern blot analysis showed that atp6-orf79 RNA of LD-cytoplasm was co-transcribed and was processed in the presence of Rf1 in the same manner as in BT-cytoplasm. Western blot analysis showed that the ORF79 peptide did not accumulate in an LD-CMS line, while ORF79 accumulated in a BT-CMS line and was diminished by Rf1. These results suggest that accumulation of ORF79 is not the cause of CMS in LD-cytoplasm and the mechanism of male-sterility induction/fertility restoration in LD-CMS is different from that in BT-CMS.