Investigation of anticancer drug lapatinib and its interaction with dsDNA by electrochemical and spectroscopic techniques
Investigation of anticancer drug lapatinib and its interaction with dsDNA by electrochemical and spectroscopic techniques
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DOI：
10.1016/j.snb.2013.12.088
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发表时间：
2014-04-01
影响因子：
8.4
通讯作者：
Uslu, Bengi
中科院分区：
文献类型：
--
作者：
Dogan-Topal, Burcu;Bozal-Palabiyik, Burcin;Uslu, Bengi
使用各种伏安技术研究了拉帕替尼 (LPT) 在玻碳电极 (GCE) 上的电氧化机制。通过循环伏安法详细测定pH和扫描速率对LPT信号的影响；结果，发现第一个和第二个峰分别受扩散和吸附控制。通过差分脉冲伏安法，LPT 在 0.76 V、0.95 V 和 1.22 V 处表现出三个阳极峰，每个峰转移两个电子。将LPT的氧化与一些含有芳香胺结构的模型化合物进行了比较。还提出了可能的电氧化途径。设计电化学生物传感器是为了显示ct-dsDNA 和LPT 之间的相互作用。为了更好地了解 LPT 和 ct-dsDNA 之间的相互作用机制，采用了光谱技术。对于电化学、紫外-可见分光光度和荧光光谱技术，LPT 和 DNA 之间的结合常数 (K) 分别计算为 6.03 x 10(5) M-1、4.20 x 10(5) M-1 和 3.50 x 10(4) M-1。最终，基于电化学和光谱方法，证实LPT可以插入双链DNA螺旋中。 (C) 2014 Elsevier B.V. 保留所有权利。
The electro-oxidation mechanism of lapatinib (LPT) at a glassy carbon electrode (GCE) was studied using various voltammetric techniques. The effects of pH and scan rate on LPT signal were determined in detail by cyclic voltammetry; as a result, the first and the second peaks were found diffusion and adsorption controlled, respectively. LPT exhibited three anodic peaks at 0.76 V, 0.95 V and 1.22 V by differential pulse voltammetry and two electrons were transferred for each peak. The oxidation of LPT was compared with some model compounds which contained aromatic amine structures. The possible electrooxidation pathway was also proposed. The electrochemical biosensor was designed in order to show the interaction between ct-dsDNA and LPT. For better understanding of the interaction mechanism between LPT and ct-dsDNA, spectroscopic techniques were performed. The binding constant (K) between LPT and DNA was calculated as 6.03 x 10(5) M-1, 4.20 x 10(5) M-1 and 3.50 x 10(4) M-1 for electrochemical, UV-vis spectrophotometric and fluorescence spectroscopic techniques, respectively. Ultimately, based on electrochemical and spectroscopic methods, it was confirmed that LPT could intercalate into the dsDNA helix. (C) 2014 Elsevier B.V. All rights reserved.