A NEW FC RECEPTOR ON MOUSE MACROPHAGES BINDING IGG
A NEW FC RECEPTOR ON MOUSE MACROPHAGES BINDING IGG
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DOI：
10.1084/jem.153.3.514
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发表时间：
1981-01-01
影响因子：
15.3
通讯作者：
YELTON, DE
中科院分区：
文献类型：
--
作者：
DIAMOND, B;YELTON, DE
绵羊红细胞单克隆抗体（SRBC）被证明可用于识别小鼠巨噬细胞上的2个Fc受体，一个用于IgG2a，一个用于IgG1和IgG2b。使用单克隆IgG3抗SRBC鉴定小鼠巨噬细胞上唯一结合IgG3的第3 Fc受体。该受体存在于原发性驻留和巯基乙酸盐诱导的腹腔巨噬细胞和一些巨噬细胞系[鼠网状细胞肉瘤5774，巨噬细胞样肿瘤P388D1]上。IgG3包被的SRBC的结合被聚集的但不是单体的IgG3抑制，并且不被IgG1、IgG2a和IgG2b聚集体抑制。它不受用胰蛋白酶或细胞松弛素B处理巨噬细胞的影响，并且在4 ℃和5 ℃时都发生。37度。C.与所有其他IgG亚类一样，IgG3介导吞噬作用。产生了携带IgG 1和IgG 2b以及IgG 2a的受体但不携带IgG 3的受体的变体巨噬细胞系。
Monoclonal antibodies to sheep erythrocytes (SRBC) proved useful in identifying 2 Fc receptors on mouse macrophages, one for IgG2a, and one for IgG1 and IgG2b. Monoclonal IgG3 anti-SRBC was used to identify a 3rd Fc receptor on mouse macrophages which binds IgG3 uniquely. This receptor is present on primary resident and thioglycolate-induced peritoneal macrophages and on some macrophage cell lines [murine reticulum cell sarcoma 5774, macrophage-like tumor P388D1]. The binding of IgG3-coated SRBC is inhibited by aggregated but not monomeric IgG3, and not by IgG1, IgG2a and IgG2b aggregates. It is unaffected by treating the macrophages with trypsin or cytochalasin B and occurs at both 4.degree. and 37.degree. C. IgG3, like all other IgG subclasses, mediates phagocytosis. A variant macrophage line which bears the receptors for IgG1 and IgG2b and for IgG2a, but not for IgG3 was generated.