Sodium action potentials in the dendrites of cerebellar Purkinje cells.
Sodium action potentials in the dendrites of cerebellar Purkinje cells.
复制标题
小脑浦肯野细胞树突中的钠动作电位。
DOI：
10.1073/pnas.89.12.5492
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发表时间：
1992
影响因子：
11.1
通讯作者：
Armstrong,CM
中科院分区：
文献类型：
--
作者：
Regehr,WG;Konnerth,A;Armstrong,CM
我们在这里报告，在小脑浦肯野细胞的轴突已被删除，正电压阶跃施加到电压钳位索马产生尖峰的有效电流。尖峰是向内的，是全或无的，具有约1 ms的持续时间，并且被河豚毒素（一种Na通道毒物）可逆地消除。从细胞到细胞，尖峰的幅度范围从4到20 nA。棘波潜伏期随着去极化步长的增大而减小。这些尖峰明显出现在电压不受控制的位置，远离索马。从这些事实，我们得出结论，浦肯野细胞树突含有足够密度的Na通道产生动作电位。平行纤维或攀缘纤维突触的激活产生类似的尖峰，表明正常输入在树突中激发Na动作电位。这些发现极大地改变了目前关于这些细胞中的树突如何响应突触输入的观点。
We report here that in cerebellar Purkinje cells from which the axon has been removed, positive voltage steps applied to the voltage-clamped soma produce spikes of active current. The spikes are inward, are all-or-none, have a duration of approximately 1 ms, and are reversibly eliminated by tetrodotoxin, a Na channel poison. From cell to cell, the amplitude of the spikes ranges from 4 to 20 nA. Spike latency decreases as the depolarizing step is made larger. These spikes clearly arise at a site where the voltage is not controlled, remote from the soma. From these facts we conclude that Purkinje cell dendrites contain a sufficient density of Na channels to generate action potentials. Activation by either parallel fiber or climbing fiber synapses produces similar spikes, suggesting that normal input elicits Na action potentials in the dendrites. These findings greatly alter current views of how dendrites in these cells respond to synaptic input.