NMR spectroscopic study of a DNA duplex with mercury-mediated T-T base pairs
NMR spectroscopic study of a DNA duplex with mercury-mediated T-T base pairs
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DOI：
10.1080/15257770600686154
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发表时间：
2006-01-01
影响因子：
1.3
通讯作者：
Ono, Akira
中科院分区：
文献类型：
--
作者：
Tanaka, Yoshiyuki;Yamaguchi, Hiroshi;Ono, Akira
最近，我们报道了T-T错配可以特异性识别Hg-II（T-Hg-II-T对形成）。为了理解T-Hg-II-T对的性质，我们记录了DNA双链体d（CGCGTTGTCC）中心点d（GGACTTCGCG）的NMR光谱，其中具有两个连续的T-T错配（Hg-II结合位点）。我们分配的H-1共振的无汞和二汞化的双链体，并进行滴定实验与汞-II通过使用1D H-1 NMR光谱。由于上述分配，我们可以确认单汞化物质的存在，因为各个组分在滴定光谱中给出了独立的NMR信号。
Recently, we reported that T-T mismatches can specifically recognize Hg-II (T-Hg-II-T pair formation). In order to understand the properties of the T-Hg-II-T pair, we recorded NMR spectra for a DNA duplex, d(CGCGTTGTCC) center dot d(GGACTTCGCG), with two successive T-T mismatches (Hg-II-binding sites). We assigned H-1 resonances for mercury-free and di-mercurated duplexes, and performed titration experiments with Hg-II by using 1D H-1 NMR spectra. Because of the above mentioned assignments, we could confirm the existence of mono-mercurated species, because individual components gave independent NMR signals in the titration spectra.