Rapid detection of Magnaporthe oryzae chrysovirus 1 dsRNA from fungal colonies on agar plates and lesions of rice blast
Rapid detection of Magnaporthe oryzae chrysovirus 1 dsRNA from fungal colonies on agar plates and lesions of rice blast
复制标题
快速检测琼脂平板真菌菌落和稻瘟病病灶中的稻瘟病病毒 1 dsRNA
DOI：
10.1007/s10327-014-0567-6
复制
发表时间：
2015
影响因子：
1.2
通讯作者：
Moriyama H and Teraoka T.
中科院分区：
文献类型：
--
作者：
Urayama S;Katoh Y;Fukuhara T;Arie T;Moriyama H and Teraoka T.
已经报道了许多植物病原真菌的与弱毒力表型相关的真菌病毒。检测真菌病毒的常用技术取决于dsRNA元件的存在。这些技术需要宿主真菌的培养、核酸的提取和dsRNA的纯化。这些程序是耗时的步骤并且使用有机溶剂。本研究建立了一种简便、快速的检测稻瘟病菌1-A型的直接一步RT-PCR方法。该方法可用于植物病原真菌侵染病斑中真菌病毒的直接检测。
Mycoviruses associated with hypovirulent phenotypes have been reported for many plant pathogenic fungi. Common techniques to detect mycoviruses depend on the presence of dsRNA elements. These techniques require cultivation of the host fungus, extraction of nucleic acids and purification of dsRNA. These procedures are time-consuming steps and use organic solvents. Here we developed a simple and rapid method detect Magnaporthe oryzae chrysovirus 1-A by direct one-step RT-PCR in samples picked using a sterilized toothpick from fungal colonies growing on plate media. This method could be applied for direct detection of mycoviruses from lesions caused by virus-infected plant pathogenic fungi.