AP-2alpha and AP-2beta regulate dorsal interneuron specification in the spinal cord.
AP-2alpha and AP-2beta regulate dorsal interneuron specification in the spinal cord.
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AP-2α 和 AP-2β 调节脊髓中的背侧中间神经元规范。
DOI：
10.1016/j.neuroscience.2016.10.055
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发表时间：
2017
期刊：
影响因子：
3.3
通讯作者：
MengshengQiu
中科院分区：
文献类型：
--
作者：
XiaofengXu;Zijing Liu;Hao Huang;Kang Zheng;Xuemei Hu;Zunyi Zhang;MengshengQiu
到目前为止，已在脊椎动物中发现了5个编码AP-2α，β，γ，δ和ε的AP-2基因，它们被报道为胚胎发育的关键调节因子。然而，AP-2家族成员在中枢神经系统(CNS)发育中的作用尚不清楚。在本研究中，我们系统地研究了AP-2基因在小鼠和鸡胚胎脊髓发育中的时空表达模式，发现AP-2α和AP-2β在有丝分裂后的背侧中间神经元中特异表达。在鸡胚胎脊髓卵电穿孔中的功能丧失分析初步证明，CaP-2α和CaP-2β分别调节背侧A类和B类中间神经元的规格。功能获得实验进一步表明，CaP-2α的错误表达，而不是CaP-2β的错误表达，能够诱导A类中间神经元的异位产生。综上所述，我们的研究表明，AP-2家族成员AP-2α和AP-2β在背侧中间神经元发育调控中具有不同的功能。
To date, five AP-2 genes that encode AP-2α, β, γ, δ and ε have been identified in vertebrates and they have been reported to be key regulators of embryonic development. However, the role of AP-2 family members in the development of central nervous system (CNS) has not been characterized. In the present study, we systematically examined the spatiotemporal expression pattern of AP-2 genes in the developing spinal cord of mouse and chick embryos and found that AP-2α and AP-2β are specifically expressed in post-mitotic dorsal interneurons. Loss-of-function analysis usingin ovoelectroporation in embryonic chick spinal cord preliminarily demonstrated that cAP-2α and cAP-2β regulates dorsal Class A and Class B interneuron specification, respectively. Gain-of-function experiments further revealed that misexpression of cAP-2α, but not cAP-2β, was able to induce the ectopic generation of Class A interneurons. Together, our studies indicated that AP-2 family members, AP-2α and AP-2β, have distinct functions in the regulation of dorsal interneuron development.