Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
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DOI：
10.1007/s00436-010-1825-y
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发表时间：
2010-05-01
影响因子：
2
通讯作者：
Kuppusamy, U. R.
中科院分区：
文献类型：
--
作者：
Chandramathi, S.;Suresh, Kumar Govind;Kuppusamy, U. R.
人类囊虫是否能入侵一直是个问题。除了一些零星的研究，如在非生物豚鼠身上进行的研究，显示了由人芽孢杆菌感染引起的宿主肠道表面入侵和粘膜炎症，没有真正的入侵文献得到证实。研究表明，透明质酸酶是在线虫寄生虫渗透到宿主皮肤和肠道时分泌的。透明质酸酶在原生动物，即溶组织内阿米巴原虫中的活性也曾被描述过。本研究旨在测定人原杆菌感染大鼠尿液样本中的透明质酸酶。尿中透明质酸酶的存在提供了人芽孢杆菌侵入结肠上皮导致细胞外基质蛋白即透明质酸（HA）降解的间接证据。透明质酸被透明质酸酶解聚，透明质酸酶可以被生物体用来相互入侵。本研究对感染人芽胞杆菌的Sprague-Dawley大鼠进行了30天的尿透明质酸酶水平监测。感染大鼠体内透明质酸酶水平在第28天和第30天显著高于接种前（P < 0.01和P < 0.05）。在此阶段，受感染粪便中的寄生虫负担也处于较高水平。感染大鼠血清中促炎因子白介素-6、白介素-8也显著升高（P < 0.05）。该研究表明，由于没有其他病原体存在，并且寄生虫的变形虫形式先前已被证明存在，因此在这一初步发现中透明质酸酶水平升高表明该生物体能够在宿主肠道中具有入侵或渗透活动。
The fact whether Blastocystis hominis can invade has always been in question. Apart from a few sporadic studies such as that done on gnotobiotic guinea pigs which showed surface invasion and mucosal inflammation of the host's intestine caused by B. hominis infection, no real documentation of invasion has been proven. Studies have shown that hyaluronidase is secreted during the penetration into the host's skin and gut by nematode parasites. Hyaluronidase activity in protozoa namely Entamoeba histolytica has also been described previously. This study attempts to determine hyaluronidase in urine samples of B. hominis-infected rats. The presence of hyaluronidase in urine provides an indirect evidence of invasion by B. hominis into colonic epithelium causing the degradation of extracellular matrix proteins namely hyaluronic acid (HA). HA is depolymerized by hyaluronidase which may be used by organisms to invade one another. In this study, the levels of urinary hyaluronidase of Sprague-Dawley rats infected with B. hominis were monitored for 30 days. Hyaluronidase levels in the infected rats were significantly higher on days 28 and 30 compared to the day before inoculation (P < 0.01 and P < 0.05, respectively). During this stage, parasitic burden in infected stools was also at a high level. Proinflammatory cytokines, interleukin-6 and interleukin-8, were also significantly higher (P < 0.05) in the serum of infected rats. The study demonstrates that since no other pathogen was present and that amoeboid forms of the parasites have been shown to exist previously, the elevated levels of hyaluronidase in this preliminary finding suggests that the organism is capable of having invasion or penetration activity in the hosts' intestine.