PHOTODYNAMIC THERAPY OF B16 PIGMENTED MELANOMA WITH LIPOSOME-DELIVERED SI(IV)-NAPHTHALOCYANINE
PHOTODYNAMIC THERAPY OF B16 PIGMENTED MELANOMA WITH LIPOSOME-DELIVERED SI(IV)-NAPHTHALOCYANINE
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DOI：
10.1111/j.1751-1097.1994.tb05048.x
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发表时间：
1994-03-01
影响因子：
3.3
通讯作者：
RODGERS, MAJ
中科院分区：
文献类型：
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作者：
BIOLO, R;JORI, G;RODGERS, MAJ
通过使用Si（IV）-萘酞菁（SiNc）作为肿瘤定位剂，测试了将光动力疗法扩展到治疗高度色素性肿瘤病变的可能性。Si（IV）-萘二甲酰亚胺在776 nm处显示强吸光度（λ = 5 × 10（5）M（-1）cm（-1）），在此黑色素吸收变得较弱。作为实验模型，我们选择B16色素性黑色素瘤皮下移植到C57 BL小鼠。注射0.5或1 mg/kg脂质体掺入的SiNc后，在24 h时观察到光敏剂在肿瘤中的最大蓄积，回收率分别为0.35和0.57 μ g/g。然而，SiNc的肿瘤靶向基本上没有显示出选择性，因为皮肤（瘤周组织）中的光敏剂浓度与我们检查的所有注射后时间的肿瘤中发现的浓度非常相似。在注射后24小时用二极管激光器的776 nm光照射装载SiNc的黑色素瘤诱导肿瘤坏死和肿瘤生长延迟。在高达260 mW cm（-2）的剂量率下，这种效应似乎是纯粹的光化学性质;在更高的剂量率下，热效应可能变得重要。
The possibility of extending photodynamic therapy to the treatment of highly pigmented neoplastic lesions was tested by using Si(IV)-naphthalocyanine (SiNc) as a tumor-localizing agent. Si(IV)-naphthalocyanine displays intense absorbance at 776 nm (epsilon = 5 x 10(5) M(-1) cm(-1)), where melanin absorption becomes weaker. As an experimental model we selected B16 pigmented melanoma subcutaneously transplanted to C57BL mice. Upon injection of 0.5 or 1 mg kg(-1) of liposome-incorporated SiNc, maximal accumulation of the photosensitizer in the tumor was observed at 24 h with recoveries of 0.35 and 0.57 mu g g(-1), respectively. However, the tumor targeting by SiNc shows essentially no selectivity, since the photosensitizer concentrations in the skin (peritumoral tissue) were very similar to those found in the tumor at all postinjection times examined by us. Irradiation of SiNc-loaded melanoma with 776 nm light from a diode laser at 24 h postinjection induces tumor necrosis and delay of tumor growth. The effect appears to be of purely photochemical nature at dose rates up to 260 mW cm(-2); at higher dose rates, thermal effects are likely to become important.