The role of ETS transcription factors in transcription and development of mouse preimplantation embryos
The role of ETS transcription factors in transcription and development of mouse preimplantation embryos
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DOI：
10.1016/j.bbrc.2006.03.192
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发表时间：
2006-06-02
影响因子：
3.1
通讯作者：
Aoki, F
中科院分区：
文献类型：
--
作者：
Kageyama, S;Liu, HL;Aoki, F
胚胎转录是创造新生命的关键过程。为了阐明胚胎转录的机制，我们研究了小鼠植入前发育过程中含成红细胞转化特异性（ETS）结构域的转录因子（TF）的表达和功能。几种ETS TF，即，elsrp 71、elf 3和spic在受精后增加，并保持在高水平，直到囊胚期。为了阐明这些TF的功能，我们使用RNA干扰进行基因抑制，这表明它们参与调控胚泡阶段的发育。此外，我们发现抑制ETS转录因子影响了e1 F-1A和oct 3/4基因的转录，这些基因的表达在胚胎中由TATA-less启动子调控。这些结果表明，ETS转录因子的功能在转录调控与TATA-少启动子在植入前胚胎，这是必不可少的植入前发育。(c)2006爱思唯尔公司All rights reserved.
Embryonic transcription is a crucial process for the creation of new life. To clarify the mechanism of embryonic transcription, we investigated the expression and function of the erythroblast transformation specific (ETS) domain containing transcription factors (TFs) during preimplantation development in mice. The expression levels of several ETS TFs, i.e., elsrp71, elf3, and spic, increased after fertilization and remained at a high level until the blastocyst stage. To clarify the function of these TFs, we performed gene suppression using RNA interference, which revealed that they were involved in regulating development to the blastocyst stage. Furthermore, we found that suppression of ETS TFs affected the transcription of e1F-lA and oct3/4 genes whose expression is regulated by TATA-less promoters in the embryos. These results suggest that ETS TFs function in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. (c) 2006 Elsevier Inc. All rights reserved.