Intersegmental recombination between the haemagglutinin and matrix genes was responsible for the emergence of a highly pathogenic H7N3 avian influenza virus in British Columbia
Intersegmental recombination between the haemagglutinin and matrix genes was responsible for the emergence of a highly pathogenic H7N3 avian influenza virus in British Columbia
复制标题
DOI：
10.1099/vir.0.80478-0
复制
发表时间：
2005-03-01
影响因子：
3.8
通讯作者：
Czub, S
中科院分区：
文献类型：
--
作者：
Pasick, J;Handel, K;Czub, S
2004年2月，不列颠哥伦比亚省爆发高致病性禽流感（HPAI）。调查表明，致病的 HPAI H7N3 病毒是从低致病性前体中突然出现的。对从指标农场分离的低致病性病毒和高致病性病毒的血凝素 (HA) 基因进行的分析表明，唯一的差异是高致病性禽流感病毒的 HA 切割位点有 21 nt 的插入。据推测，该插入很可能是同一病毒的 HA 和基质基因之间非同源重组的结果。在疫情爆发期间，总共对 37 个带有插入片段的分离株和 3 个不带插入片段的分离株进行了鉴定。这里描述的事件与 2002 年在智利发生的事件非常相似，当时另一种 H7N3 病毒的毒力转变归因于 HA 和核蛋白基因之间的非同源重组。
In February 2004 a highly pathogenic avian influenza (HPAI) outbreak erupted in British Columbia. Investigations indicated that the responsible HPAI H7N3 virus emerged suddenly from a low pathogenic precursor. Analysis of the haemagglutinin (HA) genes of the low and high pathogenic viruses isolated from the index farm revealed the only difference to be a 21 nt insert at the HA cleavage site of the highly pathogenic avian influenza virus. It was deduced that this insert most probably arose as a result of non-homologous recombination between the HA and matrix genes of the same virus. Over the course of the outbreak, a total of 37 isolates with, and 3 isolates without inserts were characterized. The events described here appear very similar to those which occurred in Chile in 2002 where the virulence shift of another H7N3 virus was attributed to non-homologous recombination between the HA and nucleoprotein genes.