Nucleotide sequence analysis of the spacer regions flanking the rat rRNA transcription unit and identification of repetitive elements.
Nucleotide sequence analysis of the spacer regions flanking the rat rRNA transcription unit and identification of repetitive elements.
复制标题
大鼠 rRNA 转录单位侧翼间隔区的核苷酸序列分析和重复元件的鉴定。
DOI：
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发表时间：
1986
影响因子：
14.9
通讯作者：
A. Hadjiolov
中科院分区：
文献类型：
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作者：
L. Yavachev;O. Georgiev;Eleonora A. Braga;T. Avdonina;A. E. Bogomolova;V. Zhurkin;V. Nosikov;A. Hadjiolov
我们通过确定位于 rRNA 转录单位上游 (2501 bp) 和下游 (4025 bp) 的大 NTS 片段的序列，研究了大鼠 rDNA 非转录间隔区 (NTS) 的组织。我们确定了四个类似 B2 的移动元素和两个 ID 移动元素。它们可以分为三对，每对的成员位于上游和下游 NTS。 ID序列与共有序列相同，而B2样元件对显示出与共有B2序列85%和50/65%的同源性。下游 NTS 的近端部分包含一个广泛分化的 SalI 串联重复区域。所分析的上游和下游NTS序列中有相当一部分是由不同类型的简单序列和长聚（嘌呤）X聚（嘧啶）链组成。这些数据表明，rRNA转录单元侧翼的大鼠rDNA NTS区域的特征是短散布序列（B2超家族）和各种简单序列的组合。
We investigated the organization of the rat rDNA non-transcribed spacer (NTS) by determining the sequence of large NTS segments located upstream (2501 bp) and downstream (4025 bp) from the rRNA transcription unit. We identified four B2-like and two ID mobile elements. They may be grouped in three pairs with the members of each pair located in the upstream and downstream NTS. The ID sequences are identical to the consensus sequence, while the pairs of B2-like elements show 85% and 50/65% homology to the consensus B2 sequence. The proximal part of the downstream NTS contains a region of widely diverged SalI tandem repeats. A considerable part of the analyzed upstream and downstream NTS sequences is constituted by different types of simple sequences and long poly(purine) X poly(pyrimidine) tracts. These data show that the rat rDNA NTS regions flanking the rRNA transcription unit are characterized by a combination of short interspersed (B2-superfamily) and various simple sequences.