EFFECT OF HOST DIET AND HINDGUT MICROBIAL COMPOSITION ON CELLULOLYTIC ACTIVITY IN THE HINDGUT OF THE AMERICAN COCKROACH, PERIPLANETA-AMERICANA
EFFECT OF HOST DIET AND HINDGUT MICROBIAL COMPOSITION ON CELLULOLYTIC ACTIVITY IN THE HINDGUT OF THE AMERICAN COCKROACH, PERIPLANETA-AMERICANA
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DOI：
10.1128/aem.60.6.1822-1826.1994
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发表时间：
1994-06-01
影响因子：
4.4
通讯作者：
OPDENCAMP, HJM
中科院分区：
文献类型：
--
作者：
GIJZEN, HJ;VANDERDRIFT, C;OPDENCAMP, HJM
在美洲大蠊蟑螂的后肠提取物中证明了以滤纸消化活性 (FPase) 和羧甲基纤维素酶 (CMCase) 测量的纤维素酶活性。 CMCase 和 FPase 的最高活性分别为 0.89 和 0.12 U.ml(-1)。当存在原生动物时，后肠群体的纤维素分解能力急剧增加，并且发现活性根据喂养方案而变化。富含纤维素的饮食诱导大量原虫数量，从而产生高纤维素酶活性。研究发现，后肠中主要原生动物 Nyctotherus ovalis 的数量与 FPase 和 CMCase 活性之间存在密切相关性。由于这种原生动物的数量也与昆虫的甲烷产生量相关，因此看来 N.utilis 负责美洲对虾后肠中发现的纤维素分解和产甲烷活性的主要部分。
Cellulase activity measured as filter paper digesting activity (FPase) and carboxymethyl cellulase (CMCase) was demonstrated in hindgut extracts of the cockroach Periplaneta americana. The highest activities measured amounted to 0.89 and 0.12 U. ml(-1) for CMCase and FPase, respectively. The cellulolytic capacity of the hindgut population increased dramatically when protozoa were present, and the activities were found to vary depending on the feeding regimen. Cellulose-rich diets induced high protozoal numbers, resulting in a high cellulase activity. A close correlation was found between the number of Nyctotherus ovalis organisms, the major protozoans in the hindgut, and both FPase and CMCase activity. Since the numbers of this protozoan also correlated with the methane production of the insect, it appears that N. avails is responsible for the major part of cellulolytic and methanogenic activity found in the hindgut of P. americana.