Sustained release of ATP encapsulated in chitosan oligosaccharide nanoparticles.
Sustained release of ATP encapsulated in chitosan oligosaccharide nanoparticles.
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封装在壳寡糖纳米颗粒中的 ATP 持续释放。
DOI：
10.1016/j.ijpharm.2010.03.050
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发表时间：
2010-06
影响因子：
5.8
通讯作者：
中科院分区：
文献类型：
--
作者：
采用W/O微乳液、化学交联和离子络合相结合的方法制备了含有ATP/CSO离子络合纳米组分的化学交联壳寡糖纳米颗粒。所得纳米颗粒直径约为110nm，表面zeta电位为20mV。纳米粒子的ATP加载效率可达40.6 ~ 69.5%。实验发现，ATP负载效率随低聚糖用量和分子量的增加而增加，随戊二醛与低聚糖摩尔比的增加而降低。化学交联CSO纳米颗粒体外ATP释放持续24h，并可受CSO的量、分子量、戊二醛与CSO的摩尔比等因素调节。CSO的分子量越大，用量越少，戊二醛与CSO的摩尔比越低，导致ATP的释放速度越慢。此外，还发现CSO纳米颗粒可被HepG-2肿瘤细胞吸收，可用于细胞内给药。
The chemical cross-linked chitosan oligosaccharide (CSO) nanoparticles containing ATP/CSO ionic complex nano-components were prepared using combination techniques of W/O miniemulsion, chemical cross-linking and ionic complexation. The resulted nanoparticles had about 110nm diameter and 20mV surface zeta potential. The ATP loading efficiencies in nanoparticles could reach up to 40.6–69.5%. It was found that the ATP loading efficiency increased with increasing the amount and the molecular weight of chitosan oligosaccharide, and decreased with increasing molar ratio of glutaraldehyde to chitosan oligosaccharide. In vitro ATP release from chemical cross-linked CSO nanoparticles could continue for 24h, and could also be adjusted by the amount and molecular weight of CSO, and the molar ratio of glutaraldehyde to CSO. The higher molecular weight and smaller amount of CSO, and the lower molar ratio of glutaraldehyde to CSO led the slower ATP release rate. Furthermore, it was also found that the CSO nanoparticles could be uptaken by HepG-2 tumor cells, and could be applied for intracellular drug delivery.
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影响因子：
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