Plasma lysophosphatidic acid level and serum autotaxin activity are increased in liver injury in rats in relation to its severity
Plasma lysophosphatidic acid level and serum autotaxin activity are increased in liver injury in rats in relation to its severity
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DOI：
10.1016/j.lfs.2007.08.013
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发表时间：
2007-09-01
期刊：
影响因子：
6.1
通讯作者：
Yatomi, Yutaka
中科院分区：
文献类型：
--
作者：
Watanabe, Naoko;Ikeda, Hitoshi;Yatomi, Yutaka
溶血磷脂酸(LPA)是一种具有多种生物学作用的脂质介质。我们已报道LPA可刺激肝星状细胞增殖，抑制肝细胞DNA合成，提示LPA可能在肝脏中起一定作用。我们发现慢性丙型肝炎患者血浆LPA水平和血清自体趋化蛋白(ATX)活性升高，但LPA及其合成酶自体趋化蛋白(ATX)的临床意义尚不清楚。为了确定血浆LPA水平和血清ATX活性是否在肝损伤中普遍存在，我们检测了不同肝损伤大鼠血液中LPA和ATX活性的变化。四氯化碳诱导的肝纤维化、二甲基亚硝胺诱导的急性肝损伤和70%肝切除术后3h血浆LPA和ATX活性均升高，且与肝纤维化程度呈正相关，与丙氨酸氨基转移酶呈正相关。慢性和急性肝损伤大鼠血浆LPA水平与血清ATX活性呈正相关。在四氯化碳诱导的肝纤维化过程中，肝组织中ATX基因的表达没有发生改变。各种肝损伤患者血浆LPA水平和血清ATX活性均随损伤程度的加重而升高。肝损伤时血中ATX和LPA升高是单纯的结果，还是损伤的原因，有待进一步阐明。(C)2007 Elsevier Inc.保留所有权利。
Lysophosphatidic acid (LPA) is a lipid mediator with multiple biological actions. We have reported that LPA stimulates hepatic stellate cell proliferation and inhibits DNA synthesis in hepatocytes, suggesting that LPA might play some role in the liver. We have found that plasma LPA level and serum autotaxin (ATX) activity were increased in patients with chronic hepatitis C. However, the clinical significance of LPA and its synthetic enzyme, autotaxin (ATX), is still unclear. To determine whether the increase of plasma LPA level and serum ATX activity might be found generally in liver injury, we examined the possible modulation of them in the blood in rats with various liver injuries. Plasma LPA level and serum ATX activity were increased in carbon tetrachloride-induced liver fibrosis correlatively with fibrosis grade, in dimethylnitrosamine-induced acute liver injury correlatively with serum alanine aminotransferase level or in 70% hepatectomy as early as 3 h after the operation. Plasma LPA level was correlated with serum ATX activity in rats with chronic and acute liver injury. ATX mRNA in the liver was not altered in carbon tetrachloride-induced liver fibrosis. Plasma LPA level and serum ATX activity are increased in various liver injuries in relation to their severity. Whether increased ATX and LPA in the blood in liver injury is simply a result or also a cause of the injury should be further clarified. (C) 2007 Elsevier Inc. All rights reserved.